Department of Otolaryngology, Head and Neck Surgery, the Second Hospital, 12510Jilin University, Changchun, Jilin, People's Republic of China.
Department of Pathophysiology, College of Basic Medical Science, 12510Jilin University, Changchun, Jilin, People's Republic of China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820962114. doi: 10.1177/1533033820962114.
To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers.
The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded.
5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin.
Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer.
探讨 5-氟尿嘧啶和姜黄素对 Hep-2 喉癌细胞的抑制和协同作用，并阐明介孔硅纳米粒子作为药物载体的效果。
使用 CCK-8 法检测 5-氟尿嘧啶和姜黄素对 Hep-2 细胞的抑制作用。采用 CompuSyn 计算 2 种药物的协同作用。流式细胞术检测 5-氟尿嘧啶和姜黄素诱导的细胞凋亡和细胞周期阻滞。将药物载入介孔纳米颗粒中。用 Western blot 检测药物处理后相关蛋白的表达。记录荷瘤 BALB/c 裸鼠腹腔注射载药介孔硅纳米颗粒后的皮下肿瘤生长情况。
5-氟尿嘧啶和姜黄素协同诱导 Hep-2 细胞凋亡和细胞周期阻滞。介孔硅纳米粒子作为药物载体增强了 5-氟尿嘧啶和姜黄素的治疗效果。
介孔硅纳米粒子有望成为增强 5-氟尿嘧啶和姜黄素对喉癌协同作用的有效药物载体。
