Shih C N, Balish E
J Clin Microbiol. 1977 Sep;6(3):249-56. doi: 10.1128/jcm.6.3.249-256.1977.
A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye in its colorless state. As the bacteria grew, they converted the dye to a visible blue color (formazan) with their reductases. The growth medium served as its own contamination control, since microbial growth and be detected by a color change before it was used for blood culture. The experiments described herein demonstrate that the composition of this medium (with the dye) provides a unique system that is able to make a reliable and rapid detection of both gram-positive and gram-negative microorganisms and yeasts (Candida albicans) commonly associated with bacteremia.
一种含有蛋白胨、葡萄糖、琥珀酸钠、乳酸钠、明胶、碳酸氢钠和蓝色四氮唑（一种指示染料）的具有特定氧化还原电位的生长培养基，置于三（羟甲基）氨基甲烷缓冲液中，用于检测血液中微生物的存在。该程序包括将血液（和细菌）引入处于无色状态的含有染料的生长培养基中。随着细菌生长，它们用还原酶将染料转化为可见的蓝色（甲臜）。该生长培养基可作为其自身的污染对照，因为在用于血液培养之前，微生物生长可通过颜色变化检测到。本文所述的实验表明，这种培养基（含染料）的组成提供了一个独特的系统，能够可靠、快速地检测出通常与菌血症相关的革兰氏阳性菌、革兰氏阴性菌和酵母（白色念珠菌）。
