Rosner R
Appl Microbiol. 1974 Aug;28(2):245-7. doi: 10.1128/am.28.2.245-247.1974.
Commercially prepared Vacutainer thioglycolate broth with SPS (TBSPS), the B-D Vacutainer culture tube with SPS (VCT), the Pfizer E-Vac hypertonic brucella broth with SPS (BBSPS), and our own Trypticase-soy hypertonic broth with SPS (TSBH) were studied by parallel culture methods for their ability to allow the survival of a wide spectrum of organisms, their ability to allow recovery of organisms, and the time required to obtain a recovery from clinical blood cultures. Organisms considered to be clinically significant were recovered from 176 blood cultures. The TSBH system produced all 176 recoveries, the BBSPS system allowed for 170 recoveries, the TBSPS recovered 131, and the VCT accounted for 106 recoveries. The time required to recover any given organism from both the BBSPS and the TSBH systems did not exceed 48 h, whereas 86 of the 131 recoveries from the TBSPS system required more than 72 h and 74 of the 106 VCT recoveries also required more than 72 h. A wide spectrum of organisms were recovered from both the BBSPS and the TSBH systems. The TBSPS system failed to allow the recovery of many streptococci and Pseudomonas spp. and the VCT system failed to recover many organisms.
采用平行培养方法，对市售含SPS的Vacutainer硫乙醇酸盐肉汤（TBSPS）、含SPS的B-D Vacutainer培养管（VCT）、辉瑞含SPS的E-Vac高渗布鲁氏菌肉汤（BBSPS）以及我们自己配制的含SPS的胰蛋白酶大豆高渗肉汤（TSBH）进行了研究，观察它们支持多种微生物存活的能力、微生物的回收率以及从临床血培养中获得阳性结果所需的时间。从176份血培养标本中分离出具有临床意义的微生物。TSBH系统实现了全部176次阳性结果，BBSPS系统有170次阳性结果，TBSPS系统有131次阳性结果，VCT系统有106次阳性结果。从BBSPS系统和TSBH系统中分离出任何一种特定微生物所需的时间均不超过48小时，而TBSPS系统的131次阳性结果中有86次需要超过72小时，VCT系统的106次阳性结果中有74次也需要超过72小时。BBSPS系统和TSBH系统均分离出了多种微生物。TBSPS系统未能分离出许多链球菌和假单胞菌属，VCT系统也未能分离出许多微生物。
