Pak J Biol Sci. 2020 Jan;23(11):1450-1455. doi: 10.3923/pjbs.2020.1450.1455.
TLR is known to regulate the immune system in cancer. TLR-7 and TLR-9 can enhance the antitumor immune system in many types of solid tumors. Cyclooxygenase-2 (COX-2) is a biomarker of inflammation. This study aimed to investigate the effect of papaya leaves extract on immune response (TLR 7, TLR 9) and inflammation (COX-2) in rats induced DMBA.
This experimental study used Sprague dawley female rats of age more less 50 days. Rats were divided into 4 groups: Negative Control (NC), Positive Control (PC), Cancer Drug Doxorubicin (DOXO) and Papaya Leaves Extract (PLE). The study was conducted for 13 weeks. DMBA induction performed for 5 weeks with administration of 2 times per week.
the expression of TLR-7 of PLE and DOXO was higher than PC groups significantly different (p<0.05). The expression of TLR-9 of PLE was higher than NC, PC and DOXO groups but not significantly different (p>0.05) while the expression of COX-2 of PLE and DOXO groups was lower than NC and PC groups but not significantly different (p>0.05).
It can be concluded that papaya leaves extract can improve the immune system and reduce inflammation. It shows that papaya leaves extract has potent as anti-cancer.
TLR 被认为可以调节癌症中的免疫系统。TLR-7 和 TLR-9 可以增强许多实体肿瘤中的抗肿瘤免疫系统。环氧化酶-2（COX-2）是炎症的生物标志物。本研究旨在探讨番木瓜叶提取物对 DMBA 诱导的大鼠免疫反应（TLR 7、TLR 9）和炎症（COX-2）的影响。
本实验研究使用年龄大于 50 天的 Sprague dawley 雌性大鼠。大鼠分为 4 组：阴性对照组（NC）、阳性对照组（PC）、癌症药物阿霉素（DOXO）和番木瓜叶提取物（PLE）。研究进行了 13 周。DMBA 诱导持续 5 周，每周给药 2 次。
PLE 和 DOXO 的 TLR-7 表达均高于 PC 组，差异有统计学意义（p<0.05）。PLE 的 TLR-9 表达高于 NC、PC 和 DOXO 组，但差异无统计学意义（p>0.05），而 PLE 和 DOXO 组的 COX-2 表达均低于 NC 和 PC 组，但差异无统计学意义（p>0.05）。
可以得出结论，番木瓜叶提取物可以改善免疫系统并减少炎症。这表明番木瓜叶提取物具有潜在的抗癌作用。
