细菌利用结构上的不完全模拟来劫持宿主相互作用组。
Bacteria use structural imperfect mimicry to hijack the host interactome.
机构信息
Gene Function and Evolution Lab, Centre for Genomic Regulation (CRG), Dr. Aiguader 88, Barcelona, Spain.
Systems Biology of Infection Lab, Department of Biochemistry and Molecular Biology, Biosciences Faculty, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
出版信息
PLoS Comput Biol. 2020 Dec 4;16(12):e1008395. doi: 10.1371/journal.pcbi.1008395. eCollection 2020 Dec.
Abstract
Bacteria use protein-protein interactions to infect their hosts and hijack fundamental pathways, which ensures their survival and proliferation. Hence, the infectious capacity of the pathogen is closely related to its ability to interact with host proteins. Here, we show that hubs in the host-pathogen interactome are isolated in the pathogen network by adapting the geometry of the interacting interfaces. An imperfect mimicry of the eukaryotic interfaces allows pathogen proteins to actively bind to the host's target while preventing deleterious effects on the pathogen interactome. Understanding how bacteria recognize eukaryotic proteins may pave the way for the rational design of new antibiotic molecules.
摘要
细菌利用蛋白质-蛋白质相互作用来感染宿主并劫持基本途径，这确保了它们的生存和增殖。因此，病原体的感染能力与其与宿主蛋白相互作用的能力密切相关。在这里，我们通过适应相互作用界面的几何形状，发现宿主-病原体相互作用组中的枢纽在病原体网络中被隔离。对真核界面的不完全模拟使病原体蛋白能够主动与宿主的靶标结合，同时防止对病原体相互作用组产生有害影响。了解细菌如何识别真核蛋白可能为新抗生素分子的合理设计铺平道路。
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本文引用的文献
1
BacFITBase: a database to assess the relevance of bacterial genes during host infection.
Nucleic Acids Res. 2020 Jan 8;48(D1):D511-D516. doi: 10.1093/nar/gkz931.
2
Protein aggregation in bacteria.
FEMS Microbiol Rev. 2020 Jan 1;44(1):54-72. doi: 10.1093/femsre/fuz026.
3
A Coordinated Response at The Transcriptome and Interactome Level is Required to Ensure Uropathogenic Survival during Bacteremia.
Microorganisms. 2019 Aug 25;7(9):292. doi: 10.3390/microorganisms7090292.
4
The fitness cost and benefit of phase-separated protein deposits.
Mol Syst Biol. 2019 Apr 8;15(4):e8075. doi: 10.15252/msb.20178075.
5
More than just a phase: the search for membraneless organelles in the bacterial cytoplasm.
Curr Genet. 2019 Jun;65(3):691-694. doi: 10.1007/s00294-018-00927-x. Epub 2019 Jan 2.
6
A thorough anion-π interaction study in biomolecules: on the importance of cooperativity effects.
Chem Sci. 2016 Feb 1;7(2):1038-1050. doi: 10.1039/c5sc01386k. Epub 2015 Jun 5.
7
Network biology discovers pathogen contact points in host protein-protein interactomes.
Nat Commun. 2018 Jun 13;9(1):2312. doi: 10.1038/s41467-018-04632-8.
8
Protein Structure Modeling with MODELLER.
Methods Mol Biol. 2017;1654:39-54. doi: 10.1007/978-1-4939-7231-9_4.
9
Protein-protein interactions leave evolutionary footprints: High molecular coevolution at the core of interfaces.
Protein Sci. 2017 Dec;26(12):2438-2444. doi: 10.1002/pro.3318. Epub 2017 Oct 25.
10
The outer membrane vesicles: Secretion system type zero.
Traffic. 2017 Jul;18(7):425-432. doi: 10.1111/tra.12488. Epub 2017 May 23.
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