The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China.
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China.
J Proteomics. 2021 Feb 10;232:104062. doi: 10.1016/j.jprot.2020.104062. Epub 2020 Dec 1.
The postgerminative development is a complex, genetically programmed process, and also the most dangerous period before the developing seedlings reach the autotrophy state. To obtain a comprehensive understanding of postgerminative development mechanism, the study focuses on an integrative analysis on transcriptome, proteome, and microRNA in cucumber seeds under drought and salt stress. Drought and salt stress caused differential expression of 4197 mRNAs, 36 microRNAs and 768 proteins compared with the control, and 827 mRNAs, 364 proteins, and 12 microRNAs were shared by the two stresses. Numerous common differentially expressed genes and proteins participated the signal transduction of plant hormone, photosynthesis, and argine and proline metabolism. We noted the correlation among nitric oxide, polyamine, proline, and ethylene metabolism, thereby helping to elucidate the role of these substances, which are derived either directly or indirectly from arginine, in the regulation of abiotic stress and provide a basis for building better network-based molecular models in further research. Above findings contribute to new and useful information regarding the common molecular mechanisms during cucumber seedling development under drought and salt stress. SIGNIFICANCE: Water scarcity and high salt are two of the most destructive and wide stress factors which limit the growth and progression of plants by affecting a variety of vital physiological and biochemical processes. Our study focuses on an integrative analysis on transcriptome, proteome, and microRNA for confirming the essential regulators as well as pathways using cucumber postgerminative development under drought and salt stress. Arginine metabolism is a vital response to abiotic stress during cucumber seed germination.
后萌发发育是一个复杂的、受基因编程控制的过程，也是发育中的幼苗达到自养状态之前最危险的阶段。为了全面了解后萌发发育的机制，本研究重点对黄瓜种子在干旱和盐胁迫下的转录组、蛋白质组和 microRNA 进行了综合分析。与对照相比，干旱和盐胁迫导致 4197 个 mRNAs、36 个 microRNAs 和 768 个蛋白质差异表达，两种胁迫共有 827 个 mRNAs、364 个蛋白质和 12 个 microRNAs。大量常见差异表达基因和蛋白质参与了植物激素、光合作用以及精氨酸和脯氨酸代谢的信号转导。我们注意到一氧化氮、多胺、脯氨酸和乙烯代谢之间的相关性，从而有助于阐明这些物质在调节非生物胁迫中的作用，这些物质直接或间接来自精氨酸，为进一步研究构建更好的基于网络的分子模型提供了依据。上述发现为黄瓜幼苗在干旱和盐胁迫下发育的共同分子机制提供了新的有用信息。意义：水分短缺和高盐是两种最具破坏性和广泛的胁迫因素，通过影响各种重要的生理和生化过程，限制了植物的生长和发育。我们的研究重点是对转录组、蛋白质组和 microRNA 进行综合分析，以确定干旱和盐胁迫下黄瓜后萌发发育的必需调控因子和途径。精氨酸代谢是黄瓜种子萌发过程中对非生物胁迫的重要反应。
