College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
College of Water Resource and Civil Engineering, China Agricultural University, Beijing 100083, China.
Int J Mol Sci. 2022 Nov 28;23(23):14911. doi: 10.3390/ijms232314911.
Cucumber is one of the most widely cultivated greenhouse vegetables, and its quality and yield are threatened by drought stress. Studies have shown that carbon dioxide concentration ([CO]) enrichment can alleviate drought stress in cucumber seedlings; however the mechanism of this [CO] enrichment effect on root drought stress is not clear. In this study, the effects of different drought stresses (simulated with 0, 5% and 10% PEG 6000, i.e., no, moderate, and severe drought stress) and [CO] (400 μmol·mol and 800 ± 40 μmol·mol) on the cucumber seedling root proteome were analyzed using the tandem mass tag (TMT) quantitative proteomics method. The results showed that after [CO] enrichment, 346 differentially accumulating proteins (DAPs) were found only under moderate drought stress, 27 DAPs only under severe drought stress, and 34 DAPs under both moderate and severe drought stress. [CO] enrichment promoted energy metabolism, amino acid metabolism, and secondary metabolism, induced the expression of proteins related to root cell wall and cytoskeleton metabolism, effectively maintained the balance of protein processing and degradation, and enhanced the cell wall regulation ability. However, the extent to which [CO] enrichment alleviated drought stress in cucumber seedling roots was limited under severe drought stress, which may be due to excessive damage to the seedlings.
黄瓜是温室中种植最广泛的蔬菜之一，其品质和产量受到干旱胁迫的威胁。研究表明，二氧化碳浓度（[CO]）富集可以缓解黄瓜幼苗的干旱胁迫；然而，这种[CO]富集对根干旱胁迫的作用机制尚不清楚。在这项研究中，采用串联质量标签（TMT）定量蛋白质组学方法，分析了不同干旱胁迫（分别用 0、5%和 10%PEG6000 模拟，即无、中度和重度干旱胁迫）和[CO]（400μmol·mol和 800±40μmol·mol）对黄瓜幼苗根蛋白质组的影响。结果表明，[CO]富集后，仅在中度干旱胁迫下发现 346 个差异积累蛋白（DAP），仅在重度干旱胁迫下发现 27 个 DAP，在中度和重度干旱胁迫下发现 34 个 DAP。[CO]富集促进了能量代谢、氨基酸代谢和次生代谢，诱导了与根细胞壁和细胞骨架代谢相关的蛋白质的表达，有效地维持了蛋白质加工和降解的平衡，增强了细胞壁调节能力。然而，在重度干旱胁迫下，[CO]富集缓解黄瓜幼苗根系干旱胁迫的程度是有限的，这可能是由于幼苗受到了过度的损伤。
