Gansu Agricultural University, State Key Laboratory of Arid Land Crop Science, Lanzhou, China.
College of Horticulture, Gansu Agricultural University, Lanzhou, China.
PeerJ. 2022 Sep 21;10:e13994. doi: 10.7717/peerj.13994. eCollection 2022.
The sucrose non-fermenting-1-related protein kinase 2 (SnRK2) is a plant-specific type of serine/threonine protein kinase that plays an important role in the physiological regulation of stress. The objective of this study was to identify and analyze the members of the SnRK2 gene family in cucumber and lay a foundation for further exploration of the mechanism of resistance to stress. Here, 12 genes were isolated from cucumber and distributed on five chromosomes, phylogenetic clustering divided these into three well-supported clades. In addition, collinearity analysis showed that the gene family underwent purifying selection pressure during evolution. genes of the same group have similar exons and conserved motifs, and intron length may be a specific imprint for the evolutionary amplification of the gene family. By predicting cis elements in the promoter, we found that the promoter region of gene members had various cis-regulatory elements in response to hormones and stress. Relative expression analysis showed that (group II) and (group III) were strongly induced by ABA, NaCl and PEG stress; whereas (group III) was not activated by any treatment. The response of group I to ABA, NaCl and PEG was weak. Furthermore, protein interaction prediction showed that multiple CsSnRK2 proteins interacted with four proteins including protein phosphatase 2C (PP2C), and it is speculated that the genes may also an independent role as a third messenger in the ABA signaling pathway. This study provides a reference for analyzing the potential function of genes in the future research.
蔗糖非发酵-1 相关蛋白激酶 2（SnRK2）是一种植物特有的丝氨酸/苏氨酸蛋白激酶，在应激的生理调节中发挥重要作用。本研究的目的是鉴定和分析黄瓜中的 SnRK2 基因家族成员，为进一步探索其应激抗性机制奠定基础。在这里，从黄瓜中分离出 12 个基因，并分布在 5 条染色体上，系统发育聚类将这些基因分为 3 个支持度较高的分支。此外，共线性分析表明，该基因家族在进化过程中经历了纯化选择压力。同一组的基因具有相似的外显子和保守基序，并且内含子长度可能是基因家族进化扩增的特定印记。通过预测启动子中的顺式元件，我们发现基因成员的启动子区域具有各种顺式调控元件，以响应激素和应激。相对表达分析表明，（第 II 组）和（第 III 组）强烈被 ABA、NaCl 和 PEG 胁迫诱导；而（第 III 组）不受任何处理的激活。第 I 组对 ABA、NaCl 和 PEG 的反应较弱。此外，蛋白质相互作用预测表明，多个 CsSnRK2 蛋白与包括蛋白磷酸酶 2C（PP2C）在内的四种蛋白质相互作用，推测这些基因也可能作为 ABA 信号通路中的独立第三信使发挥作用。本研究为未来研究分析基因的潜在功能提供了参考。
