茶藨叶蔷薇-光叶蔷薇废弃物中果胶多糖的结构特征、降血糖和免疫调节活性。
Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
机构信息
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai, 200436, PR China.
出版信息
Carbohydr Polym. 2021 Feb 1;253:117190. doi: 10.1016/j.carbpol.2020.117190. Epub 2020 Oct 9.
In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography. Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure. WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD. The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities. WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities. Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
在这项研究中，通过阴离子交换和凝胶过滤色谱法从玫瑰废料中分离出两种多糖成分，命名为 WSRP-2a 和 WSRP-2b。单糖组成、刚果红试验、FT-IR 和 NMR 光谱分析证实，这两种多糖主要由半乳糖醛酸、阿拉伯糖、半乳糖和鼠李糖组成，属于非三螺旋结构的果胶型多糖。WSRP-2a 和 WSRP-2b 的分子量分别为 56.8kD 和 23.9kD。随后的生物测定表明它们具有显著的降血糖和免疫调节活性。WSRP-2a 促进了 RAW264.7 巨噬细胞的增殖、NO 释放和细胞因子的分泌，而 WSRP-2b 则表现出更高的α-淀粉酶和α-葡萄糖苷酶抑制活性。总的来说，这些结果表明玫瑰废料生物质可以作为生物活性果胶多糖的有前途的来源。
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