Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311 Szczecin, Poland.
Department of Pharmaceutical Microbiology and Parasitology, Wrocław Medical University, Borowska 211A, 50-556 Wrocław, Poland.
Carbohydr Polym. 2021 Feb 1;253:117247. doi: 10.1016/j.carbpol.2020.117247. Epub 2020 Oct 19.
In this work, we present a novel ex situ modification of bacterial cellulose (BC) polymer, that significantly improves its ability to absorb water after drying. The method involves a single inexpensive and easy-to-perform process of BC crosslinking, using citric acid along with catalysts, such as disodium phosphate, sodium bicarbonate, ammonium bicarbonate or their mixtures. In particular, the mixture of disodium phosphate and sodium bicarbonate was the most promising, yielding significantly greater water capacity (over 5 times higher as compared to the unmodified BC) and slower water release (over 6 times as compared to the unmodified BC). Further, our optimized crosslinked BC had over 1.5x higher water capacity than modern commercial dressings dedicated to highly exuding wounds, while exhibiting no cytotoxic effects against fibroblast cell line L929 in vitro. Therefore, our novel BC biomaterial may find application in super-absorbent dressings, designed for chronic wounds with imbalanced moisture level.
在这项工作中，我们提出了一种新颖的细菌纤维素（BC）聚合物的原位修饰方法，可显著提高其干燥后吸水的能力。该方法涉及使用柠檬酸以及催化剂（如磷酸二氢钠、碳酸氢钠、碳酸氢铵或其混合物）对 BC 进行单次廉价且易于实施的交联过程。特别是，磷酸二氢钠和碳酸氢钠的混合物是最有前途的，其水容量显著增加（与未改性的 BC 相比增加了 5 倍以上），且水释放速度较慢（与未改性的 BC 相比增加了 6 倍以上）。此外，我们优化的交联 BC 的水容量比专门用于高度渗出伤口的现代商业敷料高 1.5 倍以上，而在体外对成纤维细胞系 L929 没有细胞毒性作用。因此，我们的新型 BC 生物材料可能适用于具有失衡水分水平的慢性伤口的高吸水性敷料。
