Wang Chun, Wang Bin, Aili Maimaiti, Huo Shixia, Han Meng-Ting, Aibai Silafu, Li Zhi-Jian
Department of Toxicology Laboratory, Xinjiang Institute of Traditional Uygur Medicine, Urumqi, Xinjiang, China.
College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, China.
Evid Based Complement Alternat Med. 2020 Nov 16;2020:2528617. doi: 10.1155/2020/2528617. eCollection 2020.
L. is the perennial herb of rupestris belonging to Artemisia (Compositae), which is wildly distributed in Xinjiang (China), middle Asia, and Europe. It is known to have anti-inflammatory, hepatoprotective, immune function regulation, and gastrointestinal function regulation effects. AR is used to treat digestive diseases, but the effects of AR on antifunctional dyspepsia (FD) activity have not yet been reported. In this study, we aimed to investigate the therapeutic effects of L. extract (ARE) on gastrointestinal hormones and brain-gut peptide in functional dyspepsia (FD) rats. Sixty Sprague-Dawley rats were randomly divided into 6 groups. An FD rat model was established by irregular tail clamp stimulation for 14 days except the blank group. After FD rat models, the blank group and model group were given menstruum, and the medicated rats were given corresponding medicine for 14 days. The general observations, bodyweight, and food intake were observed, and the content of serum gastrin (GAS), plasma motilin (MTL), plasma vasoactive intestinal peptide (VIP), and plasma somatostatin (SS) by the enzyme-linked immunosorbent assay was observed. The content of plasma VIP and plasma SS in the ARE group was significantly lower than in the model group, and the content of serum GAS and plasma MTL was increased in the ARE group; the GAS expression of antrum and hypothalamus was increased in the ARE group, and SS expression of antrum and hypothalamus was decreased in the ARE group by immunohistochemical detection; the results of semiquantitative reverse transcription polymerase chain reaction (RT-PCR) indicate that ARE inhibits the mRNA expression of VIP. Our results suggest that ARE can recover gastrointestinal hormone levels and regulation of the peripheral and central nervous system and alter gut peptide levels, which confirm the therapeutic effect of ARE on functional dyspepsia.
新疆一枝蒿是菊科蒿属多年生草本植物，广泛分布于中国新疆、中亚和欧洲。已知其具有抗炎、保肝、调节免疫功能和胃肠功能的作用。新疆一枝蒿用于治疗消化系统疾病，但关于其对功能性消化不良（FD）活性的影响尚未见报道。在本研究中，我们旨在探讨新疆一枝蒿提取物（ARE）对功能性消化不良（FD）大鼠胃肠激素和脑肠肽的治疗作用。将60只Sprague-Dawley大鼠随机分为6组。除空白组外，通过不规则尾夹刺激14天建立FD大鼠模型。造模后，空白组和模型组给予溶媒，给药组给予相应药物，持续14天。观察一般情况、体重和进食量，并采用酶联免疫吸附测定法观察血清胃泌素（GAS）、血浆胃动素（MTL）、血浆血管活性肠肽（VIP）和血浆生长抑素（SS）的含量。ARE组血浆VIP和血浆SS含量显著低于模型组，血清GAS和血浆MTL含量升高；免疫组化检测显示，ARE组胃窦和下丘脑GAS表达增加，胃窦和下丘脑SS表达降低；半定量逆转录聚合酶链反应（RT-PCR）结果表明，ARE抑制VIP的mRNA表达。我们的结果表明，ARE可以恢复胃肠激素水平以及外周和中枢神经系统的调节，并改变肠肽水平，这证实了ARE对功能性消化不良的治疗作用。
