Toslak Devrim, Son Taeyoon, Erol Muhammet Kazim, Kim Hoonsup, Kim Tae-Hoon, Chan R V Paul, Yao Xincheng
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
Antalya Training and Research Hospital, Department of Ophthalmology, Antalya, 07100, Turkey.
Biomed Opt Express. 2020 Oct 12;11(11):6281-6292. doi: 10.1364/BOE.406299. eCollection 2020 Nov 1.
Multispectral imaging (MSI) of the retina and choroid has increasing interest for better diagnosis and treatment evaluation of eye diseases. However, currently available MSI systems have a limited field of view (FOV) to evaluate the peripheral retina. This study is to validate trans-pars-planar illumination for a contact-mode ultra-widefield MSI system. By freeing the available pupil for collecting imaging light only, the trans-pars-planar illumination enables a portable, non-mydriatic fundus camera, with 200° FOV in a single fundus image. The trans-pars-planar illumination, delivering illumination light from one side of the eye, naturally enables oblique illumination ophthalmoscopy to enhance the contrast of fundus imaging. A broadband (104 nm) 565 nm light-emitting diode (LED) is used for validating color fundus imaging first. Four narrowband (17-60 nm) 530 nm, 625 nm, 780 nm, and 970 nm LEDs are tested for MSI. With 530 nm illumination, the fundus image reveals retinal vasculature predominantly. 625 nm and 780 nm illuminations enhance the visibility of choroidal vasculature. With further increased wavelength of 970 nm, the fundus image is predominated by large veins in the choroid, with multiple vortex ampullas observed simultaneously in a single fundus image.
视网膜和脉络膜的多光谱成像（MSI）对于眼部疾病的更好诊断和治疗评估越来越受到关注。然而，目前可用的MSI系统在评估周边视网膜时视野（FOV）有限。本研究旨在验证用于接触式超广角MSI系统的经瞳孔平面照明。通过仅释放可用瞳孔以收集成像光，经瞳孔平面照明使得能够使用便携式、无需散瞳的眼底相机，在单张眼底图像中具有200°的视野。经瞳孔平面照明从眼睛的一侧提供照明光，自然地实现了斜照式检眼镜检查以增强眼底成像的对比度。首先使用宽带（104 nm）565 nm发光二极管（LED）来验证彩色眼底成像。测试了四个窄带（17 - 60 nm）530 nm、625 nm、780 nm和970 nm的LED用于MSI。在530 nm照明下，眼底图像主要显示视网膜血管系统。625 nm和780 nm照明增强了脉络膜血管系统的可见性。随着波长进一步增加到970 nm，眼底图像主要由脉络膜中的大静脉主导，在单张眼底图像中可同时观察到多个涡静脉壶腹。
