Jara Marisa, Salcedo-Moncada Doris, Ayala Gerardo, Watanabe Romel, Alvítez-Temoche Daniel, Mayta-Tovalino Frank
Department of Rehabilitation Stomatology, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima-Peru.
Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima-Peru.
J Int Soc Prev Community Dent. 2020 Sep 28;10(5):555-560. doi: 10.4103/jispcd.JISPCD_177_20. eCollection 2020 Sep-Oct.
The objective of this study was to assess the antibacterial efficacy of Ca(OH) with iodoform versus Ca(OH) with camphorated paramonochlorophenol as intrachannel pastes on an biofilm.
The diffusion method was used in wells. The strain used was ATCC 29212. Bile esculin agar was inoculated into 60-well plates of 5 mm in diameter. Three groups were formed: Group 1: Calen PMCC (Ca(OH) + camphor paramonochlorophenol); Group 2: Metapex (Ca(OH) + iodoform); and Group 3: camphor paramonochlorophenol inoculated with as a positive control. The plates were then incubated at 37°C for 24 h. Bacterial inhibition halos were read.
Group 1 presented the highest antimicrobial efficacy with a mean of 16.2 ± 0.6 mm, whereas Group 2 only had an antimicrobial effect of 9.7 ± 1.3 mm. Finally, Group 3 only exposed to the positive control (camphor paramonochlorophenol) showed an effect of 14.6 ± 1.0 mm. The inferential analysis showed statistically significant differences between the antimicrobial effect of the three groups ( = 0.001).
Ca(OH) paste with camphor paramonochlorophenol (Calen PMCC) has a greater antibacterial action on . The iodoform-associated Ca(OH) paste (Metapex) showed significantly lower antibacterial action against ( < 0.05).
本研究旨在评估含碘仿的氢氧化钙与含樟脑对氯苯酚的氢氧化钙作为根管内糊剂对生物膜的抗菌效果。
采用平板打孔扩散法。使用的菌株为ATCC 29212。将胆汁七叶苷琼脂接种到直径5毫米的60孔板中。分为三组:第1组:Calen PMCC(氢氧化钙+樟脑对氯苯酚);第2组:Metapex(氢氧化钙+碘仿);第3组:接种樟脑对氯苯酚作为阳性对照。然后将平板在37°C下孵育24小时。读取细菌抑制圈。
第1组抗菌效果最佳,平均为16.2±0.6毫米,而第2组的抗菌效果仅为9.7±1.3毫米。最后,仅暴露于阳性对照(樟脑对氯苯酚)的第3组显示出14.6±1.0毫米的效果。推断分析显示三组抗菌效果之间存在统计学显著差异(P = 0.001)。
含樟脑对氯苯酚的氢氧化钙糊剂(Calen PMCC)对……具有更强的抗菌作用。含碘仿的氢氧化钙糊剂(Metapex)对……的抗菌作用明显较低(P<0.05)。