Lu Yan, Zhang Longyi, Chen Xuya, Zhang Qiaohong
Clinical Laboratory, Dongyang People's Hospital 60 West Wuning Road, Dongyang 322100, Zhejiang, China.
Int J Clin Exp Pathol. 2020 Nov 1;13(11):2746-2752. eCollection 2020.
Neutral Cholesterol Ester Hydrolase 1 (NCEH1) is an enzyme involved in ether lipid metabolism, and the gene is overexpressed in a variety of tumors. However, its role in pancreatic cancer remains unknown. Therefore, we compared the gene transcription data of healthy and pancreatic cancer tissues using the Cancer Genome Atlas and Genotype-Tissue Expression databases. R software (v3.6.1) was used for the differential, clinicopathological correlation, and survival analyses. We found that was overexpressed in pancreatic cancer tissues compared with that in healthy tissues ( = 1.732 e-50), and that its expression level was related to lymph node metastasis. High expression was associated with poor overall survival ( = 0.002). Using univariate and multivariate Cox regression analyses, we determined that is an independent risk factor for pancreatic cancer. Gene set enrichment analysis identified that overexpression is prominent in cell-cell adhesion junctions, pancreatic cancer, cancer-associated pathways, prostate cancer, and chronic myeloid leukemia. In contrast, low expression correlated to high oxidative phosphorylation. Thus, we conclude that may be a prognostic biomarker for pancreatic cancer.
中性胆固醇酯水解酶1（NCEH1）是一种参与醚脂代谢的酶，该基因在多种肿瘤中过表达。然而，其在胰腺癌中的作用尚不清楚。因此，我们使用癌症基因组图谱（Cancer Genome Atlas）和基因型-组织表达（Genotype-Tissue Expression）数据库比较了健康组织和胰腺癌组织的基因转录数据。使用R软件（v3.6.1）进行差异分析、临床病理相关性分析和生存分析。我们发现，与健康组织相比，NCEH1在胰腺癌组织中过表达（P = 1.732×10⁻⁵⁰），且其表达水平与淋巴结转移有关。高NCEH1表达与总体生存率低相关（P = 0.002）。通过单因素和多因素Cox回归分析，我们确定NCEH1是胰腺癌的独立危险因素。基因集富集分析表明，NCEH1过表达在细胞-细胞黏附连接、胰腺癌、癌症相关通路、前列腺癌和慢性髓性白血病中显著。相反，低NCEH1表达与高氧化磷酸化相关。因此，我们得出结论，NCEH1可能是胰腺癌的预后生物标志物。
