Chemistry Department, Faculty of Science, Eskişehir Technical University, 26470 Eskisehir, Turkey.
Soft Matter. 2021 Jan 28;17(4):1008-1015. doi: 10.1039/d0sm01734e. Epub 2020 Dec 7.
3D bioprinting allows the production of patient-specific tissue constructs with desired structural characteristics such as high resolution, controlled swelling degree, and controlled degradation behavior by mostly using hydrogels. Crosslinking of hydrogels is an essential parameter in bioprinting applications, which is beneficial for tuning structural specifications. In this study, gelatin-alginate-whey protein isolate based hydrogels have been used for 3D printing structures in a layer-by-layer fashion. These structures were cross-linked by the Amino Acid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method, which is a unique, non-invasive photosensitive cross-linking technique for protein-based mixtures. In that aim, hydrogel properties (e.g., printability, biocompatibility, rheologic and mechanical behavior) and cross-linking properties (e.g., swelling and degradation behavior) were studied. Results were compared with UV and ionic cross-linking techniques, which are the abundantly used techniques in such studies. The results showed that the ANADOLUCA method can be used for in situ cross-linking under mild conditions for the printing of bio-inks, and the proposed method can be used as an alternative for UV-based and chemical cross-linking techniques.
3D 生物打印允许通过主要使用水凝胶来生产具有所需结构特征的患者特异性组织构建体，例如高分辨率、控制溶胀度和控制降解行为。水凝胶的交联是生物打印应用中的一个重要参数，有利于调整结构规格。在这项研究中，基于明胶-海藻酸盐-乳清蛋白分离物的水凝胶已被用于以逐层方式打印结构。这些结构通过氨基酸（单体）修饰和光支撑偶联方法（ANADOLUCA）交联，这是一种独特的、非侵入性的基于蛋白质的混合物光敏感交联技术。为此，研究了水凝胶的性质（例如，可打印性、生物相容性、流变学和机械性能）和交联性质（例如，溶胀和降解行为）。结果与 UV 和离子交联技术进行了比较，这些技术在这类研究中被广泛使用。结果表明，ANADOLUCA 方法可用于温和条件下的原位交联，用于打印生物墨水，并且该方法可用作基于 UV 的和化学交联技术的替代方法。
