Molecular Sensors and Therapeutics Research Laboratory, Department of Chemistry, School of Natural Sciences, Shiv Nadar University, NH91, Tehsil Dadri, Gautam Buddha Nagar, Uttar Pradesh 201314, India.
Chemical Sciences and Technology Division, CSIR- National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Industrial Estate, PO Pappanamcode, Trivandrum, Kerala 695019, India.
J Photochem Photobiol B. 2021 Jan;214:112091. doi: 10.1016/j.jphotobiol.2020.112091. Epub 2020 Nov 24.
Formaldehyde (FA), a simple reactive carbonyl molecule, is endogenously produced in the cell at various physiological condition. At elevated level, FA causes severe cell toxicity as well as damage in macromolecules such proteins and DNA. For detecting FA in living cell, we identify a small but effective fluorescent turn on probe comprising single benzene-based orothophenylenediamine compound. Further study reveals that carboxylic group in orothophenylenediamine plays the important role in enhancing fluorescent signal than another electron withdrawing group. It is even interesting to observe the occurrence of fluorescent enhancement in glutathione (GSH) environment which is generally abundant in every cell. Our probe enables to detect FA over other bio-analytes efficiently with limit of detection of 123 nM and 355-fold of enhancement in cellular mimicking conditions. Moreover, this probe could be useful in discriminating cell that has high concentration of FA as well as GSH.
甲醛（FA）是一种简单的反应性羰基分子，在各种生理条件下在细胞内内源性产生。在高水平下，FA 会导致严重的细胞毒性以及对蛋白质和 DNA 等大分子的损伤。为了在活细胞中检测 FA，我们鉴定了一个小而有效的荧光开启探针，它由单个苯并邻苯二胺化合物组成。进一步的研究表明，邻苯二胺中的羧酸基团比另一个吸电子基团在增强荧光信号方面发挥着更重要的作用。更有趣的是，我们观察到在谷胱甘肽（GSH）环境中发生了荧光增强，而 GSH 在每个细胞中普遍丰富。我们的探针能够在细胞模拟条件下以 123 nM 的检测限和 355 倍的增强效率有效地检测 FA 以外的其他生物分析物。此外，该探针可用于区分具有高浓度 FA 和 GSH 的细胞。
