Boivin G, Morel G, Charnot Y, Meunier P J, Dubois P M
INSERM Unité 234, Faculté Alexis Carrel, Lyon.
Ann Endocrinol (Paris). 1987;48(6):481-3.
Immunoreactivity to endogenous calcitonin was studied in osteoblasts from calvaria of neonatal rats, by immunocytochemistry with the use of ultrathin sections obtained by cryoultramicrotomy. The influence of a silicon-treatment on the localization of endogenous calcitonin in osteoblasts of rats, was tested. Endogenous calcitonin-like immunoreactivity was observed in osteoblasts of calvaria of control and of silicon-treated rats, and showed a similar subcellular localization in both types of rats. Calcitonin was observed at the plasma membrane level, in the cytoplasmic matrix, and in the nucleus. The results provide immunocytological evidence for: 1) the internalization of calcitonin in osteoblasts of rat calvaria; 2) a similar localization of calcitonin in osteoblasts of control or silicon-treated rats.
采用冷冻超薄切片免疫细胞化学方法，研究新生大鼠颅骨成骨细胞对内源性降钙素的免疫反应性。检测了硅处理对大鼠成骨细胞内源性降钙素定位的影响。在对照大鼠和硅处理大鼠颅骨的成骨细胞中均观察到内源性降钙素样免疫反应性，且在两种类型的大鼠中显示出相似的亚细胞定位。降钙素在质膜水平、细胞质基质和细胞核中均有观察到。这些结果为以下方面提供了免疫细胞学证据：1）大鼠颅骨成骨细胞中降钙素的内化；2）对照或硅处理大鼠成骨细胞中降钙素的相似定位。
