Keller Birgit, Kuder Henrike, Visscher Christian, Siesenop Ute, Kamphues Josef
Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
Institute for Microbiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
J Fungi (Basel). 2020 Dec 4;6(4):337. doi: 10.3390/jof6040337.
Liquid feed is susceptible to microbiological growth. Yeasts are said to cause sudden death in swine due to intestinal gas formation. As not all animals given high yeast content feed fall ill, growth and gas formation potential at body temperature were investigated as possible causally required properties. The best identification method for these environmental yeasts should be tested beforehand. Yeasts derived from liquid diets without (LD - S) and liquid diets with maize silage (LD + S) were examined biochemically (ID32C-test) and with MALDI-TOF with direct smear (DS) and an extraction method (EX). Growth temperature and gas-forming potential were measured. With MALDI-EX, most yeast isolates were identified: most often in LD - S, and most often in LD + S, significantly more than in LD - S. Larger colonies, 58.75% of all yeast isolates, were formed at 25 °C rather than at 37 °C; 17.5% of all isolates did not grow at 37 °C at all. Most isolates formed high gas amounts within 24 h, whereas none of the , and most other isolates did. The gas pressure formed by yeast isolates varied more than tenfold. Only a minority of the yeasts were able to produce gas at temperatures common in the pig gut.
液体饲料容易滋生微生物。据说酵母会因在猪肠道内产生气体而导致猪突然死亡。由于并非所有食用高酵母含量饲料的动物都会生病，因此研究了体温下的生长和产气潜力，将其作为可能的因果相关特性。这些环境酵母的最佳鉴定方法应事先进行测试。对来自无玉米青贮饲料的液体日粮（LD - S）和含玉米青贮饲料的液体日粮（LD + S）中的酵母进行了生化检测（ID32C测试），并使用基质辅助激光解吸电离飞行时间质谱（MALDI - TOF）结合直接涂片法（DS）和提取法（EX）进行检测。测量了生长温度和产气潜力。通过MALDI - EX法，鉴定出了大多数酵母分离株：在LD - S中最常鉴定出，在LD + S中也最常鉴定出，且显著多于LD - S中的数量。58.75%的酵母分离株在25℃而非37℃形成较大菌落；17.5%的分离株在37℃根本不生长。大多数分离株在24小时内产生大量气体，而、以及大多数其他分离株则不产气。酵母分离株形成的气压变化超过十倍。只有少数酵母能够在猪肠道常见的温度下产气。
