Song Dan, Ho Chi-Tang, Zhang Xin, Wu Zufang, Cao Jinxuan
State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China.
Department of Food Science, Rutgers University, New Brunswick, NJ 08901, United States.
Food Res Int. 2020 Dec;138(Pt A):109769. doi: 10.1016/j.foodres.2020.109769. Epub 2020 Oct 6.
Host circadian rhythm and gut microbiota have a bidirectional relationship, indicating that prebiotics or prebiotic-like substance is a possible way to regulate circadian rhythm. The modulatory effect of Cyclocarya paliurus flavonoids (CPF) on the intestinal microbiota and liver clock genes of a circadian rhythm disorder mouse model was investigated in the present study. 16S rDNA sequencing analysis showed that CPF ameliorated the imbalanced intestinal microbial structure induced by circadian rhythm disorder. Compared with the constant darkness (CD) group, the ratio of the relative abundance of Firmicutes to Bacteroidetes was significantly decreased after the intervention of CPF for 4 weeks. In addition, CPF significantly alleviated the disrupted diurnal oscillation and phase shift of the specific intestinal microbes and liver clock genes induced by constant darkness. Moreover, metagenomics analysis of gut microbiota showed that the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched the most differentially expressed genes (DEGs) after CPF administration includes xenobiotics biodegradation and metabolism, carbohydrate metabolism and cell motility. The results suggested that CPF may positively regulate the gut flora disturbed by host circadian rhythm disorder, including its composition, diurnal oscillation and function, as well as affect the expression of liver clock genes, thus improving the host micro-ecology and health.
宿主昼夜节律与肠道微生物群存在双向关系，这表明益生元或类益生元物质可能是调节昼夜节律的一种方式。本研究探讨了青钱柳黄酮（CPF）对昼夜节律紊乱小鼠模型肠道微生物群和肝脏生物钟基因的调节作用。16S rDNA测序分析表明，CPF改善了昼夜节律紊乱引起的肠道微生物结构失衡。与持续黑暗（CD）组相比，CPF干预4周后，厚壁菌门与拟杆菌门相对丰度的比值显著降低。此外，CPF显著缓解了持续黑暗诱导的特定肠道微生物和肝脏生物钟基因的昼夜振荡紊乱和相位偏移。此外，肠道微生物群的宏基因组学分析表明，CPF给药后京都基因与基因组百科全书（KEGG）通路中富集差异表达基因（DEG）最多的包括外源生物降解与代谢、碳水化合物代谢和细胞运动。结果表明，CPF可能正向调节受宿主昼夜节律紊乱干扰的肠道菌群，包括其组成、昼夜振荡和功能，以及影响肝脏生物钟基因的表达，从而改善宿主微生态和健康状况。
