Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China.
Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA.
Nutrients. 2022 May 31;14(11):2308. doi: 10.3390/nu14112308.
Circadian rhythm disruption is detrimental and results in adverse health consequences. We used a multi-omics profiling approach to investigate the effects of flavonoid (CPF)-enriched diets on gut microbiota, metabolites, and hypothalamus clock genes in mice with induced circadian rhythm disruption. It was observed that CPF supplementation altered the specific composition and function of gut microbiota and metabolites induced by circadian rhythm disruption. Analysis showed that the abundance of increased, while the abundance of and displayed a significant downward trend after the CPF intervention. Correlation analysis also revealed that these gut microbes had certain correlations with the metabolites, suggesting that CPFs help the intestinal microbiota to repair the intestinal environment and modulate the release of some beneficial metabolites. Notably, single-cell RNA-seq revealed that CPF supplementation significantly regulated the expression of genes associated with circadian rhythm, myelination, and neurodegenerative diseases. Altogether, these findings highlight that CPFs may represent a promising dietary therapeutic strategy for treating circadian rhythm disruption.
昼夜节律紊乱有害，并导致不良的健康后果。我们使用多组学分析方法研究了富含类黄酮（CPF）的饮食对昼夜节律紊乱小鼠肠道微生物群、代谢物和下丘脑时钟基因的影响。结果表明，CPF 补充改变了昼夜节律紊乱诱导的肠道微生物群和代谢物的特定组成和功能。分析显示，在 CPF 干预后， 增加，而 和 的丰度呈现显著下降趋势。相关性分析还表明，这些肠道微生物与代谢物具有一定的相关性，提示 CPFs 有助于肠道微生物群修复肠道环境并调节一些有益代谢物的释放。值得注意的是，单细胞 RNA-seq 显示 CPF 补充显著调节了与昼夜节律、髓鞘形成和神经退行性疾病相关的基因表达。总之，这些发现强调了 CPFs 可能代表一种有前途的治疗昼夜节律紊乱的饮食治疗策略。
