Alnuqaydan Abdullah M
Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
J Toxicol. 2020 Nov 19;2020:8896263. doi: 10.1155/2020/8896263. eCollection 2020.
(TA) is a wild halophytic plant growing in extremely harsh environmental conditions in the deserts of Saudi Arabia. Evaluating the protective effect of the methanolic extract of different parts (fresh and dry leaves, stem, and root) of TA was determined by MTT assay using Hs27 skin fibroblasts as the cellular model. The study was designed and conducted in two sets. The first set assesses the toxicity profile of TA extracts in both concentration- and time-dependent ways on Hs27 cells. Our MTT results showed that methanolic extracts from all four parts of TA at varying doses (27.5, 55, 110, and 220 g/mL) display negligible toxicity when exposed for 4 h. However, exposure of Hs27 cells to varying doses of all four TA extracts for 24 and 48 h promotes significant 23%, 24%, 26%, and 25% ( < 0.05) and 35%, 36%, 39%, and 41% ( < 0.05) cell toxicity at 220 g/mL of all four TA extracts compared to untreated control cells. To evaluate the protection offered by TA extracts against H₂O₂, we perform a second set of experiments to preincubate Hs27 cells with the TA extracts in both dose- and time-dependent way. This is followed by 300 M hydrogen peroxide- (H₂O₂-) mediated oxidative insult for 1 h. Using MTT assay, we found that methanolic extracts of TA at different time points (4, 24, and 48 h) and higher doses (220 g/mL) provide significant protection in cell viability when challenged with HO-induced oxidative stress in Hs27 cells. The protective effect was more pronounced at 48 h and 220 g/mL and the amounts were 39%, 41%, 41%, and 44% for stem, root, fresh leaf, and dry leaf TA extracts ( < 0.05), respectively, compared to untreated cells (2-4%). Collectively, the current study demonstrates that methanolic extracts of TA contain potential bioactive compounds and offer significant protection against HO-mediated oxidative stress in Hs27 skin fibroblasts.
TA是一种野生盐生植物，生长在沙特阿拉伯沙漠极端恶劣的环境条件下。以Hs27皮肤成纤维细胞为细胞模型，通过MTT法测定TA不同部位（新鲜和干燥叶片、茎和根）甲醇提取物的保护作用。该研究分两组设计和进行。第一组以浓度和时间依赖性方式评估TA提取物对Hs27细胞的毒性特征。我们的MTT结果表明，TA所有四个部位的甲醇提取物在不同剂量（27.5、55、110和220μg/mL）下暴露4小时时显示出可忽略不计的毒性。然而，与未处理的对照细胞相比，Hs27细胞在220μg/mL的所有四种TA提取物中暴露24小时和48小时，会导致显著的细胞毒性，分别为23%、24%、26%和25%（P<0.05）以及35%、36%、39%和41%（P<0.05）。为了评估TA提取物对H₂O₂的保护作用，我们进行了第二组实验，以剂量和时间依赖性方式用TA提取物预孵育Hs27细胞。随后进行300μM过氧化氢（H₂O₂）介导的氧化损伤1小时。使用MTT法，我们发现TA的甲醇提取物在不同时间点（4、24和48小时）和较高剂量（220μg/mL）下，在Hs27细胞受到H₂O₂诱导的氧化应激挑战时，对细胞活力提供了显著的保护作用。在48小时和220μg/mL时保护作用更为明显，与未处理的细胞（2 - 4%）相比，茎、根、新鲜叶片和干燥叶片TA提取物分别为39%、41%、41%和44%（P<0.05）。总的来说，目前的研究表明，TA的甲醇提取物含有潜在的生物活性化合物，并对Hs27皮肤成纤维细胞中H₂O₂介导的氧化应激提供了显著的保护作用。
