Meireles Patrícia, Brás Daniela, Fontinha Diana, Chora Ângelo F, Serre Karine, Mendes António M, Prudêncio Miguel
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.
iScience. 2020 Nov 6;23(12):101781. doi: 10.1016/j.isci.2020.101781. eCollection 2020 Dec 18.
parasites, causative agents of malaria, scavenge host nutrients to sustain their intracellular replication. Modulation of the host's nutritional status can potentially help control infection by limiting the parasite's access to nutrients, or by boosting the immune system. Here, we show that dietary supplementation of mice employing a combination of arginine (R) with two additional amino acids, lysine (K) and valine (V), termed RKV, significantly decreases liver infection. RKV supplementation results in the elimination of parasites at a late stage of their development in the liver. Our data employing genetic knockout mouse models and depletion of specific cell populations suggest that RKV supplementation boosts the host's overall innate immune response, and that parasite elimination is dependent on MyD88 signaling in immune cells. The immunostimulatory effect of RKV supplementation opens a potential role for dietary supplementation as an adjuvant for prophylaxis or immunization strategies against infection.
疟原虫作为疟疾的病原体，会掠夺宿主营养以维持其细胞内复制。调节宿主的营养状况可能有助于通过限制寄生虫获取营养或增强免疫系统来控制感染。在此，我们表明，给小鼠饮食补充精氨酸（R）与另外两种氨基酸赖氨酸（K）和缬氨酸（V）的组合（称为RKV），可显著降低肝脏感染。补充RKV可在寄生虫在肝脏发育的后期将其清除。我们使用基因敲除小鼠模型和特定细胞群耗竭的实验数据表明，补充RKV可增强宿主的整体固有免疫反应，且寄生虫的清除依赖于免疫细胞中的MyD88信号传导。补充RKV的免疫刺激作用为饮食补充作为预防感染或免疫策略的佐剂开辟了潜在作用。
