Subbarao E K, Whitehurst N J, Waner J L
Department of Pediatrics, Oklahoma Children's Memorial Hospital, Oklahoma City 73104.
Diagn Microbiol Infect Dis. 1987 Dec;8(4):229-34. doi: 10.1016/0732-8893(87)90054-x.
Rapid diagnosis of respiratory syncytial virus (RSV) infections is based upon detection of viral antigen in cells obtained from the respiratory tract and usually employs immunofluorescence (IF) reactions or enzyme-linked immunosorbent assays (EIA). The Pathfinder EIA kit (Kallestad Diagnostics) was compared with the Abbott EIA kit by evaluating each against isolation of RSV in cell culture and detection of antigen by IF. The Pathfinder kit identified 116 of 129 culture-positive and 72 of 90 culture-negative specimens; the sensitivity was 90 percent and the specificity was 80 percent. The sensitivity of the Abbott EIA test compared to isolation of RSV in cell culture was 91% (115 of 127), and the specificity was 83% (74 of 89). Of 165 specimens evaluated by IF, the Pathfinder kit detected 97 of 105 IF-positive and 45 of 60 IF-negative specimens, giving a sensitivity of 92% and a specificity of 75%. The Abbott EIA compared similarly with IF, showing a sensitivity of 91% (98 of 108) and a specificity (42 of 54) of 78%. Visual reading of the Kallestad test resulted in a sensitivity of 92%, a specificity of 91%, positive predictive value of 95%, and negative predictive value of 86%. The Pathfinder EIA kit compared well with IF and the Abbott EIA for detection of RSV antigen but performed faster than the Abbott test and offers the option of a visual reading.
呼吸道合胞病毒（RSV）感染的快速诊断基于对从呼吸道获取的细胞中病毒抗原的检测，通常采用免疫荧光（IF）反应或酶联免疫吸附测定（EIA）。通过将Pathfinder EIA试剂盒（Kallestad诊断公司）与雅培EIA试剂盒分别与细胞培养中RSV的分离及IF法抗原检测进行比较。Pathfinder试剂盒在129份培养阳性标本中识别出116份，在90份培养阴性标本中识别出72份；灵敏度为90%，特异性为80%。与细胞培养中RSV分离相比，雅培EIA检测的灵敏度为91%（127份中的115份），特异性为83%（89份中的74份）。在165份通过IF评估的标本中，Pathfinder试剂盒在105份IF阳性标本中检测出97份，在60份IF阴性标本中检测出45份，灵敏度为92%，特异性为75%。雅培EIA与IF的比较结果相似，灵敏度为91%（108份中的98份），特异性为78%（54份中的42份）。Kallestad检测的目视判读结果为灵敏度92%、特异性91%、阳性预测值95%和阴性预测值86%。Pathfinder EIA试剂盒在检测RSV抗原方面与IF及雅培EIA相比表现良好，但比雅培检测速度更快，并且提供目视判读选项。
