叶片中生物活性化合物的研究及其生物合成金纳米粒子的抗癌活性

Investigation of bioactive compounds in leaf and anticancer activity of its biosynthesized gold nanoparticles.

作者信息

Adewale Olusola B, Anadozie Scholastica O, Potts-Johnson Sotonye S, Onwuelu Joan O, Obafemi Tajudeen O, Osukoya Olukemi A, Fadaka Adewale O, Davids Hajierah, Roux Saartjie

机构信息

Department of Chemical Sciences, Biochemistry Program, Afe Babalola University, Ado-Ekiti, P.M.B. 5454, Ado-Ekiti, 360001, Nigeria.

Department of Physiology, Nelson Mandela University, P. O. Box 77000, Port Elizabeth, 6031, South Africa.

出版信息

Biotechnol Rep (Amst). 2020 Nov 16;28:e00560. doi: 10.1016/j.btre.2020.e00560. eCollection 2020 Dec.

The development of cancer therapies has become difficult due to high metastasis, and lack of tissue selectivity, which in most cases affects normal cells. Demand for anticancer therapy is therefore increasing on daily basis. Gold nanoparticles (AuNPs) have many applications in biomedical field. Biological synthesis of AuNPs using aqueous extract of (AECR) was designed to investigate the anticancer potential. The synthesized AuNPs were characterized by UV-vis spectroscopy, high-resolution transmission electron microscopy, and Fourier transform infrared spectroscopy. The characterization results showed the formation of green AuNPs of wavelength 538 nm, and mostly spherical AuNPs with 20 ± 5 nm size. Significant anticancer activity of the AECR-AuNPs on MCF-7 and Caco-2 cells was noted at higher concentrations (125 and 250 μg/mL) during 24 and at all concentrations tested during 48 h. It can therefore be concluded that AECR leaves can mediate stable AuNPs with anticancer properties.

由于癌症的高转移性以及缺乏组织选择性（在大多数情况下会影响正常细胞），癌症治疗的发展变得困难。因此，对抗癌治疗的需求日益增加。金纳米颗粒（AuNPs）在生物医学领域有许多应用。利用[植物名称]水提取物（AECR）生物合成AuNPs旨在研究其抗癌潜力。通过紫外可见光谱、高分辨率透射电子显微镜和傅里叶变换红外光谱对合成的AuNPs进行了表征。表征结果显示形成了波长为538nm的绿色AuNPs，且大多为尺寸为20±5nm的球形AuNPs。在24小时时，较高浓度（125和250μg/mL）的AECR-AuNPs对MCF-7和Caco-2细胞具有显著的抗癌活性，在48小时的所有测试浓度下也均有此活性。因此可以得出结论，[植物名称]叶可以介导具有抗癌特性的稳定AuNPs。