Green Synthesis of Gold Nanoparticles Using Thunberg for Antibacterial and Anticancer Applications.

PURPOSE

The potential use of gold nanoparticles (AuNPs) in healthcare research has increased dramatically in recent years, owing to advancements in their synthesis techniques, including green synthesis. Although is a popular medicinal plant that harbors many bioactive phytochemicals in various parts of the world, the green synthesis of AuNPs using this precious plant is still under investigation. Therefore, this study aimed to explore the green synthesis of AuNPs from Thunberg leaves (CTT-AuNPs) and their antibacterial and cytotoxic properties.

METHODS

AuNPs were synthesized from Thunberg and characterized using various microscopic and spectroscopic techniques. A serial dilution technique was used to determine the antibacterial activity of the synthesized CTT-AuNPs against two bacterial species, and . The cytotoxicity assay was carried out in the breast cancer cell line (MCF-7), whereas the biocompatibility test was performed in the kidney cell line (293T).

RESULTS

Multiple spectroscopic characterization techniques confirmed the successful synthesis of CTT-AuNPs. The average size of CTT-AuNPs was 19.1±2.2 nm. The results of this study revealed that CTT-AuNPs showed strong antibacterial activity against and , which was further confirmed using a live/dead dual staining assay. CTT-AuNPs also significantly reduced the proliferation of MCF-7 breast cancer cells to 32.67% at 120 µg/mL after 24 hours of incubation. These green-synthesized CTT-AuNPs exhibited excellent cytobiocompatibility with 293T kidney cells. The dual staining method further confirmed the cytotoxicity and biocompatibility of CTT-AuNPs compared with chemically synthesized AuNPs.

CONCLUSION

This work will pave the way for the production of biocompatible AuNPs from Thunberg that can be used in different disease treatments.