胶体金免疫层析法检测光滑布鲁氏菌的研究进展

Development of a colloidal gold immunochromatographic test strip for detecting the smooth Brucella.

机构信息

School of Public Health, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.

Laboratory of Zoonoses, Animal Health and Epidemiology Center, No.369 Nanjing Road, 266032, Qingdao, China.

出版信息

Sci Rep. 2024 Oct 23;14(1):25068. doi: 10.1038/s41598-024-76026-4.

DOI:10.1038/s41598-024-76026-4

PMID:39443541

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11500340/

Abstract

Brucellosis, caused by Gram-negative Brucella, spreads in human and animal populations through contact with infected animals and products. Developing a rapid and sensitive detection technology for pathogen is crucial to reduce the risk of this disease transmitting between animal populations and to humans. We produced a monoclonal antibody LPS-6B5, which shows high affinity to LPS and limited cross-reactivity with other bacteria. Based on LPS-6B5, a colloidal gold immunochromatographic assay (GICA) was developed which demonstrates high sensitivity and specificity in detecting cultured B. melitensis, B. abortus and B. suis. The Gold Immunochromatographic Assay (GICA) strips exhibited the most sensitive detection limits, with a value of 7.8125 × 10 CFU/mL for Brucella melitensis, surpassing the sensitivity levels observed for Brucella abortus and Brucella suis. It is also suitable for clinical and field samples, providing a cost-effective and user-friendly alternative to traditional methods.

摘要

布鲁氏菌病由革兰氏阴性布鲁氏菌引起，通过接触受感染的动物和产品在人类和动物群体中传播。开发一种用于病原体的快速和灵敏的检测技术对于降低这种疾病在动物群体和人类之间传播的风险至关重要。我们生产了一种对 LPS 具有高亲和力且与其他细菌交叉反应有限的单克隆抗体 LPS-6B5。基于 LPS-6B5，开发了胶体金免疫层析测定法（GICA），该方法在检测培养的 B. melitensis、B. abortus 和 B. suis 时表现出高灵敏度和特异性。金免疫层析测定法（GICA）条显示出最敏感的检测极限，B. melitensis 的检测下限值为 7.8125 × 10 CFU/mL，超过了观察到的 B. abortus 和 B. suis 的灵敏度水平。它也适用于临床和现场样本，为传统方法提供了一种具有成本效益和用户友好的替代方案。