Luang-In Vijitra, Katisart Teeraporn, Konsue Ampa, Nudmamud-Thanoi Sutisa, Narbad Arjan, Saengha Worachot, Wangkahart Eakapol, Pumriw Supaporn, Samappito Wannee, Ma Nyuk Ling
Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand.
Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.
Food Sci Anim Resour. 2020 Nov;40(6):1014-1032. doi: 10.5851/kosfa.2020.e72. Epub 2020 Nov 1.
This work aimed to investigate the psychobiotic effects of six bacterial strains on the mind and behavior of male Wistar rats. The probiotic (PRO) group (n=7) were rats pre-treated with antibiotics for 7 days followed by 14-day probiotic administration, antibiotics (ANT) group (n=7) were rats treated with antibiotics for 21 days without probiotics. The control (CON) group (n=7) were rats that received sham treatment for 21 days. The six bacterial strains with probiotic properties were mostly isolated from Thai fermented foods; WS11, SK321, SK324, TRBC 3003, TBRC 7154 and subsp. TBRC 375. The probiotics were freeze-dried into powder (6×10 CFU/5 g) and administered to the PRO group via oral gavage. Behavioral tests were performed. The PRO group displayed significantly reduced anxiety level and increased locomotor function using a marble burying test and open field test, respectively and significantly improved short-term memory performance using a novel object recognition test. Antibiotics significantly reduced microbial counts in rat feces in the ANT group by 100 fold compared to the PRO group. Probiotics significantly enhanced antioxidant enzymatic and non-enzymatic defenses in rat brains as assessed using catalase activity and ferric reducing antioxidant power assay, respectively. Probiotics also showed neuroprotective effects with less pyknotic cells and lower frequency of vacuolization in cerebral cortex. This multi-strain probiotic formulation from Thai fermented foods may offer a potential to develop psychobiotic-rich functional foods to modulate human mind and behaviors.
这项研究旨在探究六种细菌菌株对雄性Wistar大鼠心理和行为的心理益生菌效应。益生菌（PRO）组（n = 7）的大鼠先用抗生素预处理7天，然后进行14天的益生菌给药；抗生素（ANT）组（n = 7）的大鼠用抗生素治疗21天，未使用益生菌。对照组（CON）（n = 7）的大鼠接受了21天的假处理。六种具有益生菌特性的细菌菌株大多从泰国发酵食品中分离得到；WS11、SK321、SK324、TRBC 3003、TBRC 7154和亚种TBRC 375。将益生菌冻干成粉末（6×10 CFU/5 g），通过灌胃法给予PRO组。进行了行为测试。PRO组分别使用大理石埋藏试验和旷场试验显示焦虑水平显著降低和运动功能增强，并使用新物体识别试验显示短期记忆表现显著改善。与PRO组相比，抗生素使ANT组大鼠粪便中的微生物数量显著减少了100倍。分别使用过氧化氢酶活性和铁还原抗氧化能力测定法评估，益生菌显著增强了大鼠大脑中的抗氧化酶和非酶防御能力。益生菌还显示出神经保护作用，大脑皮层中固缩细胞较少，空泡化频率较低。这种来自泰国发酵食品的多菌株益生菌制剂可能具有开发富含心理益生菌的功能性食品以调节人类心理和行为的潜力。
