Department of Integrated Traditional Chinese and Western Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province 450000, China.
Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, P.O. Box-60169, Riyadh 11545, Saudi Arabia.
Int J Biol Macromol. 2021 Feb 28;171:198-207. doi: 10.1016/j.ijbiomac.2020.12.037. Epub 2020 Dec 10.
Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability. In this investigation, magnetic cores (FeO) were synthesized using a fabrication method involving coprecipitation of Fe and Fe. Then the magnetic nanoparticles were encapsulated by chitosan layers. In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent. The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM. In the biological part of the present study, the cell viability of FeO, HAuCl, and FeO@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2. The IC50 of FeO@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively. Thereby, the best cytotoxicity results of our FeO@CS/AuNPs were observed in the case of the HCT 116 cell line. Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
壳聚糖是一种线性多糖，是一种无毒的生物活性聚合物，由于其易于修饰和可生物降解等功能特性，具有广泛的应用。在本研究中，通过共沉淀法合成了磁性核（FeO）。然后，用壳聚糖层包裹磁性纳米粒子。下一步，使用柠檬酸作为天然还原剂，合成了具有球形形貌和尺寸为 20-30nm 的磁性-金复合纳米粒子。使用傅里叶变换红外光谱（FT-IR）、电感耦合等离子体分析（ICP 分析）、场发射扫描电子显微镜（FESEM）、能谱仪（EDS）、X 射线衍射（XRD）、透射电子显微镜（TEM）、X 射线光电子能谱（XPS）和振动样品磁强计（VSM）等多种先进技术对材料的形态和物理化学性质进行了测定。在本研究的生物学部分，FeO、HAuCl 和 FeO@CS/AuNPs 对人结直肠癌细胞系即 Ramos.2G6.4C10、HCT-8[HRT-18]、HCT 116 和 HT-29、人胃癌细胞系即 MKN45、AGS 和 KATO III 以及人胰腺癌细胞系即 PANC-1、AsPC-1 和 MIA PaCa-2 的细胞活力非常低。FeO@CS/AuNPs 对 Ramos.2G6.4C10、HCT-8[HRT-18]、HCT 116、HT-29、MKN45、AGS、KATO III、PANC-1、AsPC-1 和 MIA PaCa-2 细胞系的 IC50 值分别为 385、429、264、286、442、498、561、513、528 和 425μg/mL。因此，我们的 FeO@CS/AuNPs 在 HCT 116 细胞系中表现出最佳的细胞毒性。显然，目前的纳米粒子可能在不久的将来用于治疗多种类型的胃十二指肠癌，特别是结肠癌、胃癌和胰腺癌。
