氧化铜纳米颗粒对绿藻、细菌和甲壳类动物系统的细胞毒理学评估。
Cytotoxicological evaluation of copper oxide nanoparticles on green algae, bacteria and crustacean systems.
作者信息
Janani B, Al Farraj Dunia A, Raju Lija L, Elshikh Mohamed S, Alkubaisi Noorah A, Thomas Ajith M, Das Arunava, Sudheer Khan S
机构信息
Nanobiotechnology Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India.
Department of Botany and Microbiology, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia.
出版信息
J Environ Health Sci Eng. 2020 Oct 10;18(2):1465-1472. doi: 10.1007/s40201-020-00561-1. eCollection 2020 Dec.
PURPOSE
Copper oxide (CuO) nanoparticles (NPs) have been utilized in several industries including textile, consumer products, medical, automobiles etc. The discharge of industrial effluents in environment increased the probability of CuO NPs contamination in the ecosystem.
METHODS
The present investigation used CuO NPs to determine the toxic effect on species, fresh water algae isolated from natural pond, bacterial species and and a crustacean species .
RESULTS
The NPs average diameter and zeta potential was estimated to be 45 ± 3 nm and 29 ± 1.78 mV respectively. The results showed that 0.1 µg/mL CuO NPs showed the growth inhibition of 47 ± 2% on sp. after 5 days of incubation. The CuO NPs also showed toxic effect to bacterial systems such as and and crustacean system . Further, there was an increased lipid peroxidation and generation of reactive oxygen species (ROS) in algal cells observed up on NPs exposure. The exposure of NPs suppressed the antioxidant defense system. The amount of glutathione was reduced after the exposure of NPs.
CONCLUSION
The study suggested the role of ROS in toxicity of algal and bacterial systems. The present study pointed out the potent toxicity of CuO NPs to the organisms present in the aquatic environment.
目的
氧化铜（CuO）纳米颗粒（NPs）已应用于多个行业，包括纺织、消费品、医疗、汽车等。工业废水排放到环境中增加了生态系统中CuO NPs污染的可能性。
方法
本研究使用CuO NPs来确定对从天然池塘分离的淡水藻类物种、细菌物种以及甲壳类物种的毒性作用。
结果
NPs的平均直径和zeta电位分别估计为45±3nm和29±1.78mV。结果表明，在孵育5天后，0.1μg/mL的CuO NPs对某物种显示出47±2%的生长抑制。CuO NPs对诸如某细菌系统以及甲壳类系统也显示出毒性作用。此外，在暴露于NPs后，观察到藻类细胞中脂质过氧化增加和活性氧（ROS）的产生。NPs的暴露抑制了抗氧化防御系统。暴露于NPs后谷胱甘肽的量减少。
结论
该研究表明ROS在藻类和细菌系统毒性中的作用。本研究指出了CuO NPs对水生环境中生物的潜在毒性。
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