一个用于药物发现的多样化全球真菌文库。
A diverse global fungal library for drug discovery.
作者信息
Niu Guodong, Annamalai Thirunavukkarasu, Wang Xiaohong, Li Sheng, Munga Stephen, Niu Guomin, Tse-Dinh Yuk-Ching, Li Jun
机构信息
Department of Biological Sciences, Florida International University, Miami, FL, United States of America.
Department of Chemistry and Biochemistry, Florida International University, Miami, FL, United States of America.
出版信息
PeerJ. 2020 Nov 27;8:e10392. doi: 10.7717/peerj.10392. eCollection 2020.
BACKGROUND
Secondary fungal metabolites are important sources for new drugs against infectious diseases and cancers.
METHODS
To obtain a library with enough diversity, we collected about 2,395 soil samples and 2,324 plant samples from 36 regions in Africa, Asia, and North America. The collection areas covered various climate zones in the world. We examined the usability of the global fungal extract library (GFEL) against parasitic malaria transmission, Gram-positive and negative bacterial pathogens, and leukemia cells.
RESULTS
Nearly ten thousand fungal strains were isolated. Sequences of nuclear ribosomal internal transcribed spacer (ITS) from 40 randomly selected strains showed that over 80% were unique. Screening GFEL, we found that the fungal extract from was able to block transmission to , and the fungal extract from was able to kill myelogenous leukemia cell line K562. We also identified a set of candidate fungal extracts against bacterial pathogens.
背景
次生真菌代谢产物是抗传染病和癌症新药的重要来源。
方法
为获得一个具有足够多样性的文库，我们从非洲、亚洲和北美的36个地区收集了约2395份土壤样本和2324份植物样本。收集区域覆盖了世界上各种气候带。我们检测了全球真菌提取物文库（GFEL）对寄生性疟疾传播、革兰氏阳性和阴性细菌病原体以及白血病细胞的适用性。
结果
分离出近一万株真菌菌株。对随机选择的40株菌株的核糖体内部转录间隔区（ITS）序列分析表明，超过80%的菌株是独特的。通过筛选GFEL，我们发现来自[具体来源1]的真菌提取物能够阻断[具体疟疾传播途径]的传播，来自[具体来源2]的真菌提取物能够杀死髓性白血病细胞系K562。我们还鉴定出一组针对细菌病原体的候选真菌提取物。
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