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使用聚合酶链反应检测法检测设拉子省消费的未巴氏杀菌乳制品中各物种的流行情况。

Prevalence of species in unpasteurized dairy products consumed in Shiraz province using PCR assay.

作者信息

Abdali Fargol, Hosseinzadeh Saeid, Berizi Enayat, Pourmontaseri Maryam

机构信息

Nutrition Research Center, Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

出版信息

Mol Biol Res Commun. 2020 Sep;9(3):117-121. doi: 10.22099/mbrc.2020.37381.1506.

DOI:10.22099/mbrc.2020.37381.1506
PMID:33313331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7727767/
Abstract

The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of () and () in unpasteurized dairy products consumed in Shiraz province. In this study conducted in 2016, 238 unpasteurized dairy products including 48 raw milk, 48 yogurt, 46 cheeses, 48 dough and 48 ice cream samples, were purchased from the retail market in Shiraz province and were examined by a specific PCR assay. This study showed positive 5/04% out of 238 unpasteurized dairy products including 9 out of 48 (18/75%) raw milk samples and 3 out of 48 (6.25%) yogurt samples). Contamination was not detected in samples of dough, cheese and traditional ice cream. The results also showed that among 12 positive samples, 6 samples were contaminated with (including 4 milk samples and 2 yogurt samples), 2 samples were contaminated with (including 2 Milk samples) and 4 samples were contaminated simultaneously with and (including 3 milk samples and 1 yogurt sample). The present study suggests the unpasteurized dairy products as the major sources of brucellosis in Shiraz province, South of Iran; thus, to prevent brucellosis in human, the consumption of pasteurized milk and dairy products is highly recommended.

摘要

食用被布鲁氏菌污染的牛奶和未经巴氏杀菌的乳制品是布鲁氏菌病传播给人类的最重要途径之一。本研究的主要目的是确定设拉子省消费的未经巴氏杀菌的乳制品中()和()的流行情况。在这项于2016年进行的研究中,从设拉子省的零售市场购买了238份未经巴氏杀菌的乳制品,包括48份生牛奶、48份酸奶、46份奶酪、48份面团和48份冰淇淋样品,并通过特定的PCR检测进行检查。这项研究显示,在238份未经巴氏杀菌的乳制品中,阳性率为5.04%,其中包括48份生牛奶样品中的9份(18.75%)和48份酸奶样品中的3份(6.25%)。在面团、奶酪和传统冰淇淋样品中未检测到污染。结果还显示,在12份阳性样品中,6份样品被(包括4份牛奶样品和2份酸奶样品)污染,2份样品被(包括2份牛奶样品)污染,4份样品同时被和污染(包括3份牛奶样品和1份酸奶样品)。本研究表明,未经巴氏杀菌的乳制品是伊朗南部设拉子省布鲁氏菌病的主要来源;因此,为预防人类布鲁氏菌病强烈建议食用巴氏杀菌的牛奶和乳制品。

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本文引用的文献

1
Epidemiology of Milk-Borne Diseases.经牛奶传播疾病的流行病学
J Food Prot. 1983 Jul;46(7):637-649. doi: 10.4315/0362-028X-46.7.637.
2
Brucella melitensis and Mycobacterium tuberculosis depict overlapping gene expression patterns induced in infected THP-1 macrophages.羊种布鲁氏菌和结核分枝杆菌在感染的THP-1巨噬细胞中呈现出重叠的基因表达模式。
Iran J Vet Res. 2015 Fall;16(4):368-73.
3
Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep's milk cheese.从生羊奶奶酪中分离的金黄色葡萄球菌菌株的毒力因子和遗传变异性。
Int J Food Microbiol. 2012 Feb 1;153(1-2):53-7. doi: 10.1016/j.ijfoodmicro.2011.10.015. Epub 2011 Oct 29.
4
Brucellosis - regionally emerging zoonotic disease?布鲁氏菌病——区域性新发人畜共患病?
Croat Med J. 2010 Aug;51(4):289-95. doi: 10.3325/cmj.2010.51.289.
5
Brucellosis.布鲁氏菌病
N Engl J Med. 2005 Jun 2;352(22):2325-36. doi: 10.1056/NEJMra050570.
6
PCR as a diagnostic tool for brucellosis.聚合酶链反应作为布鲁氏菌病的诊断工具。
Vet Microbiol. 2002 Dec 20;90(1-4):435-46. doi: 10.1016/s0378-1135(02)00228-6.
7
Single-tube, nested PCR for the diagnosis of human brucellosis in Kuwait.用于科威特人布鲁氏菌病诊断的单管巢式聚合酶链反应
Ann Trop Med Parasitol. 2002 Jun;96(4):397-403. doi: 10.1179/000349802125001203.
8
An overview of human brucellosis.人类布鲁氏菌病概述。
Clin Infect Dis. 1995 Aug;21(2):283-9; quiz 290. doi: 10.1093/clinids/21.2.283.
9
Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR.通过聚合酶链反应(PCR)鉴别流产布鲁氏菌1型、2型和4型、羊种布鲁氏菌、绵羊布鲁氏菌及猪种布鲁氏菌1型。
J Clin Microbiol. 1994 Nov;32(11):2660-6. doi: 10.1128/jcm.32.11.2660-2666.1994.