Tian Ye-Han, Peng Hai-Ying, Wang De-Hao, Li Xiao-Fang, He Bang-Ling, Gao Ke-Xiang
College of Plant Protection, Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Tai'an 271000, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2020 Oct;31(10):3255-3266. doi: 10.13287/j.1001-9332.202010.037.
strain Q2 is a kind of beneficial microbe originally separated from the rhizosphere of healthy cucumber. In this study, we evaluated the biocontrol potential of strain Q2 against four soil-borne diseases by plate confrontation culture, potting in the greenhouse. We further estimated the control efficacy of strain Q2 combined metam-sodium fumigation against wilt of bitter gourd in the field. The mechanism of strain Q2 controlling bitter gourd wilt and regulating soil microbial community was examined by plate dilution culture, high throughput sequencing and quantitative PCR. The results showed that strain Q2 could efficiently reduce disease incidence of wilt of bitter gourd, potato stem canker, black shank of tobacco and black root rot of tobacco in the green house. Its biocontrol efficacy on black shank of tobacco and bitter gourd wilt was 75.3% and 63.4%, respectively. Biocontrol efficacy of strain Q2 on bitter gourd wilt was 51.0% in the artificial disease nursery inoculated pathogen of bitter gourd wilt, while the control efficacy of strain Q2 combined with soil fumigation technology was more than 80% in the same experiment condition. Strain Q2 application and soil fumigation altered soil microbial community composition and recovery trend. Metam-sodium fumigation significantly reduced the abundances of and causal agent of bitter gourd wilt. Strain Q2 further suppressed the efficient recovery trend of the pathogen. After application of strain Q2, was enriched in soil, as well as the beneficial microbes involved in the suppression of such as and . Overall, after soil fumigation, biocontrol efficacy of strain Q2 on soil-borne diseases such as wilt could attribute to the formation of beneficial microbial communities in soil and inhibition of strain Q2 on growth and development of .
菌株Q2是一种最初从健康黄瓜根际分离出的有益微生物。在本研究中，我们通过平板对峙培养、温室盆栽评估了菌株Q2对四种土传病害的生防潜力。我们还进一步评估了菌株Q2与威百亩熏蒸联合对田间苦瓜枯萎病的防治效果。通过平板稀释培养、高通量测序和定量PCR研究了菌株Q2防治苦瓜枯萎病和调节土壤微生物群落的机制。结果表明，菌株Q2能有效降低温室中苦瓜枯萎病、马铃薯茎溃疡病、烟草黑胫病和烟草黑根腐病的发病率。其对烟草黑胫病和苦瓜枯萎病的生防效果分别为75.3%和63.4%。在接种苦瓜枯萎病病原菌的人工病圃中，菌株Q2对苦瓜枯萎病的生防效果为51.0%，而在相同实验条件下，菌株Q2与土壤熏蒸技术联合的防治效果超过80%。施用菌株Q2和土壤熏蒸改变了土壤微生物群落组成和恢复趋势。威百亩熏蒸显著降低了苦瓜枯萎病病原菌的丰度。菌株Q2进一步抑制了病原菌的有效恢复趋势。施用菌株Q2后，土壤中 以及参与抑制 的有益微生物如 和 得到富集。总体而言，土壤熏蒸后，菌株Q2对苦瓜枯萎病等土传病害的生防效果可归因于土壤中有益微生物群落的形成以及菌株Q2对 生长发育的抑制作用。
