Tian Yehan, Zhao Yang, Fu Xuesong, Yu Chengming, Gao Kexiang, Liu Huixiang
College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Shandong, China.
Front Microbiol. 2021 Oct 8;12:724842. doi: 10.3389/fmicb.2021.724842. eCollection 2021.
wilt is an important disease of many food crops and often causes serious damages to yield and food quality. Consequently, numerous studies mainly focused on exploring the control strategy for as well as the mechanism of interaction between the and other beneficial soil microorganisms. In this study, we have screened and identified an efficient biocontrol strain from the soil with infection of f. sp. (referred to as Fom), Q2 (referred to as TpQ2), which could be effective to reduce relative abundance of the rhizospheric Fom, leading to a significant decrease of wilt disease incidence in bitter gourd during the greenhouse and field trails. TpQ2 can reduce the relative abundance of rhizospheric Fom through inhibition of growth and development of Fom. During the co-cultivation of TpQ2 and Fom, we confirmed that TpQ2 could significantly suppress the growth and development of Fom through disturbing the normal hyphae shape and function of the cell walls of Fom secreting cell wall-degrading enzymes and suppression of the expression of cell wall biosynthesis genes, such as . In the meantime, TpQ2 showed a strong negative correlation with in soil and positive correlation with beneficial indigenous microorganisms that had significant negative correlation with populations, such as , and To summarize, TpQ2 has a good biocontrol efficacy on wilt of bitter gourd. The biocontrol mechanisms of TpQ2 on wilt are complex and diverse.
枯萎病是许多粮食作物的重要病害，常对产量和食品质量造成严重损害。因此，众多研究主要集中在探索其防治策略以及该病原菌与其他有益土壤微生物之间的相互作用机制。在本研究中，我们从感染了尖孢镰刀菌古巴专化型（Fusarium oxysporum f. sp. cubense，简称为Fom）的土壤中筛选并鉴定出一株高效生防菌株，命名为Q2（简称为TpQ2），它能有效降低根际Fom的相对丰度，从而在温室和田间试验中显著降低苦瓜枯萎病的发病率。TpQ2可通过抑制Fom的生长发育来降低根际Fom的相对丰度。在TpQ2与Fom的共培养过程中，我们证实TpQ2可通过扰乱Fom的正常菌丝形态和细胞壁功能、分泌细胞壁降解酶以及抑制细胞壁生物合成基因（如[具体基因名称未给出]）的表达，从而显著抑制Fom的生长发育。与此同时，TpQ2与土壤中的Fom呈强负相关，与与Fom种群呈显著负相关的有益土著微生物（如[具体微生物名称未给出]、[具体微生物名称未给出]和[具体微生物名称未给出]）呈正相关。综上所述，TpQ2对苦瓜枯萎病具有良好的生防效果。TpQ2对枯萎病的生防机制复杂多样。
