Laboratory of Functional Physiology and Valorization of Bio-Resources (LR23ES08), University of Jendouba, Higher Institute of Biotechnology of Béja, Béja, Tunisia.
Laboratory of Sylvo-Pastoral Resources, Institution of Agricultural Research and Higher Education (IRESA), University of Jendouba, Sylvo- Pastoral Institute of Tabarka, Tabarka, Tunisia.
Neurogastroenterol Motil. 2024 Nov;36(11):e14892. doi: 10.1111/nmo.14892. Epub 2024 Aug 8.
Between food and medicine, nutraceuticals are widely used in human health for the prevention and treatment of various diseases. This study aims to determine the cytoprotective effects of Anethum gravelons fruit extract (AGFAE) on castor oil-induced diarrhea in rats due to its phytochemical and antioxidant properties.
Male rats were divided into six groups of six animals each: Control (C), Castor oil (CO), CO + different doses of AGFAE (50, 100, and 200 mg/kg, b.w., p.o.), and the CO + loperamide group (LOP, 10 mg/kg, b.w., p.o.).
In vitro, the chemical composition of aqueous Dill fruit extract showed strong antioxidant activity, with a high content of total polyphenols, flavonoids, and tannins. In our in vivo studies, pre-treatment with AGFAE reduced malondialdehyde and hydrogen peroxide levels and maintained normal activity of enzymatic and non-enzymatic antioxidants in the gastric and intestinal mucosa. In addition, we found that AGFAE prophylaxis improved the stability of many plasma biochemical parameters altered by castor oil intoxication, such as C-reactive protein concentrations and alkaline phosphatase activities.
CONCLUSIONS & INFERENCES: We suggest that AGFAE phenolic compounds had significant protection against diarrhea involving several mechanisms such as reducing hypersecretion, peristaltic, inflammation, and preserving the endogenous antioxidant levels.
在食品和药物之间，植物药被广泛用于人类健康，以预防和治疗各种疾病。本研究旨在确定莳萝果实提取物（AGFAE）对蓖麻油诱导的大鼠腹泻的细胞保护作用，因为它具有植物化学和抗氧化特性。
雄性大鼠分为六组，每组六只动物：对照组（C）、蓖麻油（CO）、CO+不同剂量的 AGFAE（50、100 和 200mg/kg，bw，po）和 CO+洛哌丁胺组（LOP，10mg/kg，bw，po）。
体外，水莳萝果实提取物的化学成分显示出很强的抗氧化活性，总多酚、类黄酮和单宁含量高。在我们的体内研究中，AGFAE 预处理降低了丙二醛和过氧化氢的水平，并维持了胃和肠黏膜中酶和非酶抗氧化剂的正常活性。此外，我们发现 AGFAE 预防可改善蓖麻油中毒改变的许多血浆生化参数的稳定性，如 C 反应蛋白浓度和碱性磷酸酶活性。
我们认为 AGFAE 酚类化合物对涉及减少过度分泌、蠕动、炎症和维持内源性抗氧化水平的几种机制的腹泻具有显著的保护作用。
