Unit of Functional Physiology and Valorization of Bio-Resources, University of Jendouba, Higher Institute of Biotechnology of Béja, Béja, Tunisia.
Faculty of Sciences of Bizerte, University of Carthage, Zarzouna, Tunisia.
J Med Food. 2021 Oct;24(10):1100-1112. doi: 10.1089/jmf.2020.0202. Epub 2021 Apr 19.
Medicinal plants were used to prevent and treat numerous gastrointestinal disorders owing, in part, to their antioxidant capacity. The protective effects of fruit aqueous extract (DKFAE) against castor oil (CO)-induced diarrhea was studied. The antioxidant and antibacterial properties were investigated using colorimetric and biochemical analyses. , 60 male rats were divided into 6 groups of 10 animals each ( = 10): control (C), CO, CO+various doses of DKFAE (100, 200, and 400 mg/kg, , ), and CO+loperamide (LOP, 10 mg/kg, , ).The DKFAE was rich in tannins and showed interesting antioxidant and antibacterial activities. The liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) analysis resulted in the identification of 16 phenolic compounds, among which quinic acid was the main one. The study showed that diarrhea was accompanied by an oxidative stress status as measured by an increase of lipid peroxidation, a decrease of glutathione and thiol group levels, as well as antioxidant enzyme activity depletion, such as glutathione peroxidase, superoxide dismutase, and catalase. The DKFAE administration significantly decreased the gastrointestinal transit in a dose-dependent manner. Besides, DKFAE protected against CO-induced diarrhea and intestinal fluid accumulation. Interestingly, DKFAE pretreatment counteracted all the oxidative stress status deregulation induced by CO intoxication. fruit could be suggested for its strong protective effect against CO-induced acute diarrhea, which could be explained, in part, to its antimicrobial and antioxidant properties.
药用植物被用于预防和治疗多种胃肠道疾病，部分原因是它们具有抗氧化能力。本研究旨在探讨 果实水提物（DKFAE）对蓖麻油（CO）诱导的腹泻的保护作用。采用比色法和生化分析研究了其抗氧化和抗菌特性。将 60 只雄性大鼠分为 6 组，每组 10 只（n=10）：对照组（C）、CO、CO+不同剂量的 DKFAE（100、200 和 400mg/kg）和 CO+洛哌丁胺（LOP，10mg/kg）。DKFAE 富含单宁，表现出有趣的抗氧化和抗菌活性。液相色谱-电喷雾电离质谱（LC-ESI-MS）分析结果鉴定出 16 种酚类化合物，其中以奎宁酸为主。研究表明，腹泻伴随着氧化应激状态，表现为脂质过氧化增加、谷胱甘肽和巯基水平降低以及抗氧化酶活性耗竭，如谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶。DKFAE 给药可显著降低胃肠道转运，呈剂量依赖性。此外，DKFAE 可预防 CO 诱导的腹泻和肠道液体积聚。有趣的是，DKFAE 预处理可逆转 CO 中毒引起的所有氧化应激状态失调。因此， 果实可因其对 CO 诱导的急性腹泻的强烈保护作用而被推荐，这部分可以归因于其抗菌和抗氧化特性。
