Xiao Jian, Zhou Yajiao, Ye Mengqi, An Ying, Wang Kangning, Wu Qiuji, Song Liwan, Zhang Junwen, He Huacheng, Zhang Qianwen, Wu Jiang
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, P. R. China.
College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325027, P. R. China.
Adv Healthc Mater. 2021 Mar;10(6):e2001591. doi: 10.1002/adhm.202001591. Epub 2020 Dec 15.
Imbalance of metal ions in the wound microenvironment is a key factor that leads to delayed wound healing. However, single metal administration to enhance wound repair is usually not enough due to the overlapping nature of the wound healing phases. Herein, a facile freeze-thawing strategy is developed to incorporate chitosan/ions hydrogel into medical gauzes to realize on-demand release of multiple ions to accelerate wound healing. In vitro study reveals that the gauzes can temporally release multiple metal ions on demand, and the released metal ions show effectiveness in killing bacteria and expediting cell migration. In vivo studies demonstrate that the metal ions loaded gauzes can efficiently enhance infected wound healing. Further histological analysis find that these metal ion-loaded gauzes accelerate wound healing by promoting granulation formation, collagen deposition and maturation, re-epithelization, angiogenesis, and inhibiting inflammation via regulating the expression of inflammatory factors (e.g., tumor necrosis factor-α) and polarization of macrophages. Thus, this novel metal ions delivery system has great potential in infected tissue repair and antibacterial applications.
伤口微环境中金属离子的失衡是导致伤口愈合延迟的关键因素。然而，由于伤口愈合阶段的重叠性质，单一施用金属以促进伤口修复通常是不够的。在此，开发了一种简便的冻融策略，将壳聚糖/离子水凝胶掺入医用纱布中，以实现多种离子的按需释放，从而加速伤口愈合。体外研究表明，纱布可以根据需要适时释放多种金属离子，并且释放的金属离子在杀菌和促进细胞迁移方面表现出有效性。体内研究表明，负载金属离子的纱布可以有效地促进感染伤口的愈合。进一步的组织学分析发现，这些负载金属离子的纱布通过促进肉芽形成、胶原蛋白沉积和成熟、再上皮化、血管生成，并通过调节炎症因子（如肿瘤坏死因子-α）的表达和巨噬细胞极化来抑制炎症，从而加速伤口愈合。因此，这种新型金属离子递送系统在感染组织修复和抗菌应用中具有巨大潜力。
