Gelli Rita, Di Pompo Gemma, Graziani Gabriela, Avnet Sofia, Baldini Nicola, Baglioni Piero, Ridi Francesca
Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, via della Lastruccia 3-13, 50019 Sesto Fiorentino, Italy.
BST Biomedical Science and Technologies Lab, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
ACS Biomater Sci Eng. 2020 Oct 12;6(10):5538-5548. doi: 10.1021/acsbiomaterials.0c00983. Epub 2020 Sep 17.
In the framework of new materials for orthopedic applications, Magnesium Phosphate-based Cements (MPCs) are currently the focus of active research in biomedicine, given their promising features; in this field, the loading of MPCs with active molecules to be released in the proximity of newly forming bone could represent an innovative approach to enhance the in vivo performances of the biomaterial. In this work, we describe the preparation and characterization of MPCs containing citrate, an ion naturally present in bone which presents beneficial effects when released in the proximity of newly forming bone tissue. The cements were characterized in terms of handling properties, setting time, mechanical properties, crystallinity, and microstructure, so as to unravel the effect of citrate concentration on the features of the material. Upon incubation in aqueous media, we demonstrated that citrate could be successfully released from the cements, while contributing to the alkalinization of the surroundings. The cytotoxicity of the materials toward human fibroblasts was also tested, revealing the importance of a fine modulation of released citrate to guarantee the biocompatibility of the material.
在骨科应用新材料的框架下，磷酸镁基骨水泥（MPCs）因其具有前景的特性，目前是生物医学领域积极研究的焦点；在该领域，将MPCs与活性分子负载，使其在新形成骨附近释放，可能代表一种创新方法，以提高生物材料的体内性能。在这项工作中，我们描述了含柠檬酸盐的MPCs的制备和表征，柠檬酸盐是骨中天然存在的一种离子，当在新形成的骨组织附近释放时具有有益作用。对骨水泥的操作性能、凝结时间、力学性能、结晶度和微观结构进行了表征，以揭示柠檬酸盐浓度对材料特性的影响。在水性介质中孵育后，我们证明柠檬酸盐可以从骨水泥中成功释放，同时有助于使周围环境碱化。还测试了材料对人成纤维细胞的细胞毒性，揭示了精细调节释放的柠檬酸盐以确保材料生物相容性的重要性。
