Wu Xiaopei, Dai Honglian, Yu Suchun, Zhao Yanan, Long Yanpiao, Li Wenqin, Tu Jing
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China.
Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory, Xianhu Hydrogen Valley, Foshan 528200, China.
ACS Biomater Sci Eng. 2020 Nov 9;6(11):6299-6308. doi: 10.1021/acsbiomaterials.0c00929. Epub 2020 Oct 13.
The development of bioactive bone cement is still a challenge for vascularized bone regeneration. Citrate participated in multiple biological processes, such as energy metabolism, osteogenesis, and angiogenesis. However, it is difficult to obtain a thorough and comprehensive understanding on osteogenic effects of exogenous citrate from different experimental conditions and treatment methods. In this study, by using a magnesium calcium phosphate cement (MCPC) matrix, we investigated the dual effect of exogenous citrate on osteogenesis and angiogenesis. Our studies show that citrate elevates the osteogenic function of osteoblasts under low doses and the angiogenic function of vascular endothelial cells under a broader dose range. These findings furnish a new strategy for regulating angiogenesis and osteogenic differentiation by administration of citrate in MCPC, driving the development of bioactive bone repair materials.
生物活性骨水泥的研发仍是血管化骨再生面临的一项挑战。柠檬酸盐参与了多个生物学过程，如能量代谢、骨生成和血管生成。然而，从不同的实验条件和处理方法中，很难对外源性柠檬酸盐的成骨作用获得全面而深入的认识。在本研究中，我们使用磷酸镁钙骨水泥（MCPC）基质，研究了外源性柠檬酸盐对骨生成和血管生成的双重作用。我们的研究表明，低剂量时柠檬酸盐可提高成骨细胞的成骨功能，在更宽的剂量范围内可提高血管内皮细胞的血管生成功能。这些发现为通过在MCPC中施用柠檬酸盐来调节血管生成和成骨分化提供了一种新策略，推动了生物活性骨修复材料的发展。
