Chopra Vianni, Thomas Jijo, Sharma Anjana, Panwar Vineeta, Kaushik Swati, Sharma Shivani, Porwal Konica, Kulkarni Chirag, Rajput Swati, Singh Himalaya, Jagavelu Kumaravelu, Chattopadhyay Naibedya, Ghosh Deepa
Institute of Nanoscience and Technology, Habitat Centre, Sector 64, Phase 10., Mohali 160062, Punjab, India.
Division of Endocrinology, CSIR-Central Drug Research Institute, Council of Scientific and Industrial Researchs, Lucknow 226031, U.P., India.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6710-6725. doi: 10.1021/acsbiomaterials.0c00370. Epub 2020 Nov 6.
Repair of critical size bone defects is a clinical challenge that usually necessitates the use of bone substitutes. For successful bone repair, the substitute should possess osteoconductive, osteoinductive, and vascularization potential, with the ability to control post-implantation infection serving as an additional advantage. With an aim to develop one such substitute, we optimized a zinc-doped hydroxyapatite (HZ) nanocomposite decorated on reduced graphene oxide (rGO), termed as GHZ, and demonstrated its potential to augment the bone repair. The biocompatible composite displayed its osteoconductive potential in biomineralization studies, and its osteoinductive property was confirmed by its ability to induce mesenchymal stem cell (MSC) differentiation to osteogenic lineage assessed by in vitro mineralization (Alizarin red staining) and expression of osteogenic markers including runt-related transcription factor 2 (RUNX-2), alkaline phosphatase (ALP), type 1 collagen (COL1), bone morphogenic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). While the potential of GHZ to support vascularization was displayed by its ability to induce endothelial cell migration, attachment, and proliferation, its antimicrobial activity was confirmed using . Biocompatibility of GHapZ was demonstrated by its ability to induce bone regeneration and neovascularization . These results suggest that GHZ nanocomposites can be exploited for a range of strategies in developing orthopedic bone grafts to accelerate bone regeneration.
修复临界尺寸的骨缺损是一项临床挑战，通常需要使用骨替代物。为了成功进行骨修复，替代物应具备骨传导性、骨诱导性和血管化潜力，能够控制植入后感染则是一项额外优势。为了开发这样一种替代物，我们优化了一种负载锌的羟基磷灰石（HZ）纳米复合材料，其负载于还原氧化石墨烯（rGO）上，称为GHZ，并证明了其促进骨修复的潜力。这种生物相容性复合材料在生物矿化研究中显示出其骨传导潜力，其骨诱导特性通过其诱导间充质干细胞（MSC）向成骨谱系分化的能力得到证实，这通过体外矿化（茜素红染色）以及包括 runt 相关转录因子 2（RUNX - 2）、碱性磷酸酶（ALP）、I 型胶原蛋白（COL1）、骨形态发生蛋白 - 2（BMP - 2）、骨钙素（OCN）和骨桥蛋白（OPN）在内的成骨标志物的表达来评估。虽然 GHZ 支持血管化的潜力通过其诱导内皮细胞迁移、附着和增殖的能力得以体现，但其抗菌活性通过……得以证实。GHapZ 的生物相容性通过其诱导骨再生和新血管形成的能力得以证明。这些结果表明，GHZ 纳米复合材料可用于开发骨科骨移植的一系列策略，以加速骨再生。
