Zhang Yi Fan, Li Ga Long, Gao Xiao, Zhang Huan, Zhang Ting Bin, Jiao Wang Bo, Chen Xiao Yong, Zhu Wen Jing, Liu Xiao Li, Fan Hai Ming
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China.
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education; The College of Life Sciences, School of Medicine, Northwest University, Xi'an 710069, China.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6652-6660. doi: 10.1021/acsbiomaterials.0c01443. Epub 2020 Nov 18.
Magnetic hyperthermia (MH) mediated by magnetic nanoparticles is one of the most promising antitumor modalities. The past several decades have witnessed great progress for MH antitumor therapy in scientific trials and clinic applications since it was initially advanced by Gilchrist et al. The ultimate object of MH in vivo is to efficiently kill cancer cells, and hence, it is of great importance to develop an optimized cellular MH method to evaluate the therapeutic efficiency in vitro. In this study, we systematically studied the considerable affecting factors of cancer cell-killing efficiency during the cellular MH process, including the region of cell vessel positioned inside the alternating magnetic field copper coil, the magnetic field amplitude, the types of cancer cells, etc. Taking all these into account, we introduced a method for standardizing the cellular MH process to evaluate the cell-killing efficiency.
由磁性纳米颗粒介导的磁热疗（MH）是最具前景的抗肿瘤方法之一。自吉尔克里斯特等人最初提出以来，在过去几十年里，磁热疗抗肿瘤疗法在科学试验和临床应用方面取得了巨大进展。磁热疗在体内的最终目标是有效杀死癌细胞，因此，开发一种优化的细胞磁热疗方法以在体外评估治疗效率非常重要。在本研究中，我们系统地研究了细胞磁热疗过程中影响癌细胞杀伤效率的诸多因素，包括置于交变磁场铜线圈内的细胞容器区域、磁场强度、癌细胞类型等。综合考虑所有这些因素，我们引入了一种标准化细胞磁热疗过程以评估细胞杀伤效率的方法。
