Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, United States.
Reproductive Medicine, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, China.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6701-6709. doi: 10.1021/acsbiomaterials.0c01468. Epub 2020 Nov 13.
The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.
迫切需要开发基于人类细胞的系统来替代使用啮齿动物或二维细胞培养来研究肾毒性。人类尿液来源的干细胞在肾细胞外基质诱导下在三维（3-D）培养中分化为肾小管上皮细胞。3-D 类器官中 CYP2E1 和 KIM-1 的水平在响应丙酮和顺铂时显著增加。这种 3-D 培养系统为肾毒性筛选和研究提供了一种替代工具。
