Grudzień Joanna, Jarosz Magdalena, Kamiński Kamil, Kobasa Mirosława, Wolski Karol, Kozieł Marcin, Pisarek Marcin, Sulka Grzegorz D
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Nanomaterials (Basel). 2020 Dec 13;10(12):2499. doi: 10.3390/nano10122499.
The main focus of this work was to establish a correlation between surface topography and chemistry and surface colonization by lactic acid bacteria. For this reason, we chose gold substrates with different surface architectures (i.e., smooth and nanorough) that were characterized by atomic force microscopy (AFM), electron scanning microscopy (SEM), and X-ray diffractometry (XRD). Moreover, to enhance biocompatibility, we modified gold substrates with polymeric monolayers, namely cationic dextran derivatives with different molar masses. The presence of those layers was confirmed by AFM, infrared spectroscopy (IR), and X-ray photoelectron spectroscopy (XPS). In order to determine the adhesion abilities of non-modified and modified gold surfaces, we tested three lactic acid bacteria (LAB) strains (i.e., GG, and 299v). We have shown that surface roughness influences the surface colonization of bacteria, and the most significant impact on the growth was observed for the GG strain. What is more, covering the gold surface with a molecular polymeric film by using the layer-by-layer (LbL) method allows additional changes in the bacterial growth, independently on the used strain. The well-being of the bacteria cells on tested surfaces was confirmed by using selective staining and fluorescence microscopy. Finally, we have determined the bacterial metabolic activity by measuring the amount of produced lactic acid regarding the growth conditions. The obtained results proved that the adhesion of bacteria to the metallic surface depends on the chemistry and topography of the surface, as well as the specific bacteria strain.
这项工作的主要重点是建立表面形貌与化学性质以及乳酸菌在表面定殖之间的关联。因此，我们选择了具有不同表面结构（即光滑和纳米粗糙）的金基底，通过原子力显微镜（AFM）、电子扫描显微镜（SEM）和X射线衍射仪（XRD）对其进行表征。此外，为了提高生物相容性，我们用聚合物单层修饰金基底，即不同摩尔质量的阳离子葡聚糖衍生物。通过AFM、红外光谱（IR）和X射线光电子能谱（XPS）证实了这些层的存在。为了确定未修饰和修饰的金表面的粘附能力，我们测试了三种乳酸菌（LAB）菌株（即GG、和299v）。我们已经表明，表面粗糙度会影响细菌在表面的定殖，并且观察到GG菌株对生长的影响最为显著。此外，通过逐层（LbL）方法用分子聚合物膜覆盖金表面会使细菌生长发生额外变化，这与所使用的菌株无关。通过使用选择性染色和荧光显微镜证实了测试表面上细菌细胞的健康状况。最后，我们通过测量在不同生长条件下产生的乳酸量来确定细菌的代谢活性。所得结果证明，细菌对金属表面的粘附取决于表面的化学性质和形貌以及特定的细菌菌株。
