Department of Microbiology and Food Technology, Faculty of Agriculture and Biotechnology, University of Science and Technology, Al. Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland.
Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Molecules. 2020 Dec 11;25(24):5874. doi: 10.3390/molecules25245874.
Due to the demonstrated intestinal microbial transformation of strawberry ellagitannins (ET) into bioactive metabolites, in the current study on rats, we hypothesised that the dietary addition of a strawberry ET-rich extract (S-ET) to a high-fat diet (HFD) would attenuate disturbances in the redox and lipid status as well as in the inflammatory response. We randomly distributed 48 Wistar rats into six groups and used two-way analysis of variance (ANOVA) to assess the effects of two main factors-diet type (standard and high-fat) and ET dosage (without, low, and 3× higher)-applied to rats for 4 weeks. In relation to the hypothesis, irrespective of the dosage, the dietary application of ET resulted in the desired attenuating effects in rats fed a HFD as manifested by decreased body weight gain, relative mass of the epididymal pad, hepatic fat, oxidized glutathione (GSSG), triglycerides (TG), total cholesterol (TC), and thiobarbituric acid-reactive substances (TBARS) concentrations as well as desired modifications in the blood plasma parameters. These beneficial changes were enhanced by the high dietary addition of ET, which was associated with considerably higher concentrations of ET metabolites in the urine and plasma of rats. The results indicated that S-ET could be effectively used for the prevention and treatment of metabolic disturbances associated with obesity, dyslipidaemia, redox status imbalance, and inflammation.
由于草莓鞣花单宁（ET）在肠道微生物转化为生物活性代谢物方面的作用已得到证实，因此在本项针对大鼠的研究中，我们假设在高脂肪饮食（HFD）中添加富含 ET 的草莓提取物（S-ET）将减轻氧化还原和脂质状态以及炎症反应的紊乱。我们将 48 只 Wistar 大鼠随机分为六组，并使用双因素方差分析（ANOVA）评估两种主要因素（饮食类型（标准和高脂肪）和 ET 剂量（无、低和高 3 倍））对大鼠的影响，持续 4 周。根据假设，无论剂量如何，ET 的饮食应用均可在 HFD 喂养的大鼠中产生所需的减弱作用，表现为体重增加减少，附睾垫的相对质量，肝脂肪，氧化型谷胱甘肽（GSSG），甘油三酸酯（TG），总胆固醇（TC）和硫代巴比妥酸反应物质（TBARS）浓度降低以及血浆参数的所需改变。高剂量 ET 的添加增强了这些有益的变化，这与大鼠尿液和血浆中 ET 代谢物的浓度显著升高有关。结果表明，S-ET 可有效用于预防和治疗与肥胖，血脂异常，氧化还原状态失衡和炎症相关的代谢紊乱。
