Dziedzinska Radka, Krzyzankova Miroslava, Bena Marcel, Vasickova Petra
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Viruses. 2020 Dec 12;12(12):1429. doi: 10.3390/v12121429.
Hepatitis E virus (HEV) is the etiological agent behind hepatitis E infection. Domestic pigs and wild boars are the main animal reservoirs of HEV. Very few papers describe HEV infection in goats and sheep. As the data pertaining to the presence of HEV virus in the milk of small ruminants in Europe are lacking, the aim of this paper was to examine a representative number of milk samples from these animals. The detection of HEV genome (HEV RNA) was performed using reverse transcriptase real-time polymerase chain reaction (RT-qPCR). HEV RNA was found in 2.8% of the examined samples. Positivity ranged from 10 to 10 genome equivalents/mL (GE/mL) with a median of 9.99 × 10 GE/mL. On the basis of these results, the milk of small ruminants could represent a source of HEV infection to consumers.
戊型肝炎病毒（HEV）是戊型肝炎感染背后的病原体。家猪和野猪是HEV的主要动物宿主。很少有论文描述山羊和绵羊的HEV感染情况。由于欧洲缺乏关于小型反刍动物乳汁中存在HEV病毒的数据，本文的目的是检测这些动物的代表性乳汁样本数量。使用逆转录实时聚合酶链反应（RT-qPCR）进行HEV基因组（HEV RNA）的检测。在所检测的样本中，2.8%发现了HEV RNA。阳性范围为10至10基因组当量/毫升（GE/mL），中位数为9.99×10 GE/mL。基于这些结果，小型反刍动物的乳汁可能是消费者感染HEV的一个来源。
