Ashfaq Shomaila, Ahmad Mushtaq, Zafar Muhammad, Sultana Shazia, Bahadur Saraj, Ahmed Sidra N, Gul Saba, Nazish Moona
Quaid-i-Azam University, Department of Plants Sciences, Faculty of Plant Sciences, University Road, 45320 Islamabad, Pakistan.
The Women university Multan, Department of botany, Faculty of Botany, 66000 Multan, Pakistan.
An Acad Bras Cienc. 2020 Dec 11;92(3):e20181221. doi: 10.1590/0001-3765202020181221. eCollection 2020.
The pollen micro-morphology of family Solanaceae from the different phytogeographical region of Pakistan has been assessed. In this study, thirteen species belonging to ten genera of Solanaceae have been studied using light and scanning electron microscopy for both qualitative and quantitative features. Solanaceae is a eurypalynous family and a significant variation was observed in pollen size, shape, polarity and exine sculpturing. Examined plant species includes, Brugmansia suaveolens, Capsicum annuum, Cestrum parqui, Datura innoxia, Solanum lycopersicum, Nicotiana plumbaginifolia, Petunia hybrida, Physalis minima, Solanum americanum, Solanum erianthum, Solanum melongena, Solanum surattense and Withania somnifera. The prominent pollen type is tricolporate and shed as a monad. High pollen fertility reflects that observed taxa are well-known in the study area. Based on the observed pollen traits a taxonomic key was developed for the accurate and quick identification of species. Principal Component Analysis was performed that shows some morphological features are the main characters in the identification. Cluster Analysis was performed that separate the plant species in a cluster. The findings highlight the importance of Palyno-morphological features in the characterization and identification of Solanaceous taxa. It is concluded that both LM and SEM significantly play a key role in correct identification of taxa studied.
对来自巴基斯坦不同植物地理区域的茄科植物的花粉微观形态进行了评估。在本研究中，利用光学显微镜和扫描电子显微镜对茄科10个属的13个物种的定性和定量特征进行了研究。茄科是一个花粉类型多样的科，在花粉大小、形状、极性和外壁纹饰方面观察到了显著差异。所研究的植物物种包括，光滑天使喇叭、辣椒、夜香树、无刺曼陀罗、番茄、垂花烟草、矮牵牛、极小酸浆、美洲茄、毛花茄、茄子、刺天茄和睡茄。主要的花粉类型是三孔沟型，以单粒形式散落。高花粉育性表明所观察的分类群在研究区域内是广为人知的。基于观察到的花粉特征，制定了一个分类检索表，用于准确快速地鉴定物种。进行了主成分分析，结果表明一些形态特征是鉴定中的主要特征。进行了聚类分析，将植物物种分成不同的类群。研究结果突出了孢粉形态特征在茄科分类群表征和鉴定中的重要性。得出的结论是，光学显微镜和扫描电子显微镜在正确鉴定所研究的分类群方面都起着关键作用。
