Mu Huiying, Miki Koji, Harada Hiroshi, Tanaka Kouki, Nogita Kohei, Ohe Kouichi
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Laboratory of Cancer Cell Biology, Radiation Biology Center, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
ACS Sens. 2021 Jan 22;6(1):123-129. doi: 10.1021/acssensors.0c01926. Epub 2020 Dec 17.
Photoacoustic (PA) imaging is an emerging molecular imaging modality that complements fluorescence imaging and enables high resolution within deep tissue. Fluorescence/PA multimodality imaging would be a powerful technique to extract more comprehensive information from targets than traditional single-modality imaging. In this paper, we developed a new pH-activatable sensor, , which is applicable to both fluorescence and PA detection. was derived from our previous near-infrared pH-sensing platform, in which substitution with a bulky phenyl group and functionalization with a cRGD peptide remarkably improved the sensor's biocompatibility with attenuated dye aggregation. The multimodality imaging applications of were demonstrated in cultured cells and cancer-xenografted mice with rapid kinetics and high sensitivity and specificity, which relies on cRGD-facilitated tumor targeting, probe accumulation and subsequent activation in the acidic organelles after endocytosis.
光声（PA）成像是一种新兴的分子成像方式，它是荧光成像的补充，能够在深层组织内实现高分辨率成像。荧光/光声多模态成像将成为一种强大的技术，与传统的单模态成像相比，它能够从目标中提取更全面的信息。在本文中，我们开发了一种新型的pH可激活传感器，其适用于荧光和光声检测。该传感器源自我们之前的近红外pH传感平台，其中用一个庞大的苯基进行取代并用cRGD肽进行功能化显著提高了传感器的生物相容性，同时减少了染料聚集。该传感器的多模态成像应用在培养细胞和癌症异种移植小鼠中得到了验证，具有快速动力学以及高灵敏度和特异性，这依赖于cRGD促进的肿瘤靶向、探针积累以及内吞后在酸性细胞器中的后续激活。
