Hsu Shan-Ling, Chou Wen-Yi, Hsu Chieh-Cheng, Ko Jih-Yang, Jhan Shun-Wun, Wang Ching-Jen, Lee Meng-Shiou, Hsu Tsai-Chin, Cheng Jai-Hong
Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833, Taiwan.
Department of Orthopedic Surgery, Sports Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833, Taiwan.
Biomedicines. 2020 Dec 15;8(12):614. doi: 10.3390/biomedicines8120614.
Osteoporosis (OP) causes bone loss and weakness, increasing the risk of bone fracture. In this study, rats were divided into Sham, OP, SW(F) (0.25 mJ/mm with 1600 impulses to the left medial femur), and SW(T) (0.25 mJ/mm with 1600 impulses to the left medial tibia). The bone strength results following SW(T) were better than SW(F) in the modulus, extension at peak load, handleability, and strain at break. SW(T) had the best prevention for bone loss in both lower limbs of ovariectomized (OVX) rats. The cartilage cellular matrixes of both knees were improved in SW(T) and SW(F) compared to that of OP. Serum bone morphogenetic protein 2 (BMP2) in rats undergoing SW(T) or SW(F) was significantly improved compared to that in Sham and OP. The expressions of BMP2, BMP4, and SMAD family member 4 (Smad4) in addition to the Wnt family member 3A (Wnt3a) and Cyclin D1 signaling key factors were significantly induced in the cartilage of both knees by shockwave (SW). SW(T) presented the best efficacy to induce serum BMP2 to prevent bone loss from both lower limbs. Here, we display the protective effects of SW therapy to induce BMP2, BMP4, Smad4, Wnt3a, and Cyclin D1 signaling factors for cartilage loss in both knees of OVX rats.
骨质疏松症（OP）会导致骨质流失和骨强度下降，增加骨折风险。在本研究中，将大鼠分为假手术组、骨质疏松症组、SW(F)组（对左股骨内侧给予0.25 mJ/mm的能量，1600次脉冲）和SW(T)组（对左胫骨内侧给予0.25 mJ/mm的能量，1600次脉冲）。SW(T)组后的骨强度结果在模量、峰值负荷下的伸长、可操作性和断裂应变方面均优于SW(F)组。SW(T)对去卵巢（OVX）大鼠双下肢的骨质流失具有最佳的预防作用。与骨质疏松症组相比，SW(T)组和SW(F)组双膝的软骨细胞基质均有所改善。与假手术组和骨质疏松症组相比，接受SW(T)或SW(F)治疗的大鼠血清骨形态发生蛋白2（BMP2）显著改善。冲击波（SW）显著诱导了双膝软骨中BMP2、BMP4、SMAD家族成员4（Smad4）以及Wnt家族成员3A（Wnt3a）和细胞周期蛋白D1信号关键因子的表达。SW(T)在诱导血清BMP2以预防双下肢骨质流失方面表现出最佳疗效。在此，我们展示了SW疗法对诱导BMP2、BMP4、Smad4、Wnt3a和细胞周期蛋白D1信号因子对OVX大鼠双膝软骨损失的保护作用。
