Colonization of Germ-Free Piglets with Mucinolytic and Non-Mucinolytic Strains Isolated from the Intestine of Wild Boar and Their Interference with Typhimurium.

Non-typhoidal serovars are worldwide spread foodborne pathogens that cause diarrhea in humans and animals. Colonization of gnotobiotic piglet intestine with porcine indigenous mucinolytic RP36 strain and non-mucinolytic strain RP37 and their interference with Typhimurium infection were compared. Bacterial interferences and impact on the host were evaluated by clinical signs of salmonellosis, bacterial translocation, goblet cell count, mRNA expression of mucin 2, villin, claudin-1, claudin-2, and occludin in the ileum and colon, and plasmatic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both bifidobacterial strains colonized the intestine comparably. Neither RP36 nor RP37 strains effectively suppressed signs of salmonellosis. Both strains suppressed the growth of . Typhimurium in the ileum and colon. The mucinolytic RP36 strain increased the translocation of . Typhimurium into the blood, liver, and spleen.