驱动蛋白超家族成员18B（KIF18B）促进结肠腺癌中的细胞增殖。
Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma.
作者信息
Zhao Fei, Feng Yunzhang, Zhang Xueqiang, Liu Xiaohui, Li Aili
机构信息
Department of Gastrointestinal Surgery, HanDan Central Hospital, Heibei, People's Republic of China.
HanDan Central Hospital President's Office, HanDan Central Hospital, Heibei, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Dec 11;12:12769-12778. doi: 10.2147/CMAR.S261894. eCollection 2020.
BACKGROUND
The role of kinesin superfamily proteins (KIFs) has been reported in a variety of tumors and KIFs contributed to the proliferation of cancer cells. But few studies were focus on colon adenocarcinoma.
METHODS
Through bioinformatics analysis and immunohistochemistry (IHC) assays, the expression of KIF18B in colon adenocarcinoma tissues was determined. Stable KIF18B-depleted cell lines were constructed using lentivirus-mediated shRNA of KIF18B. Cell colony formation assay and CCK8 assay were performed to assess cell proliferation degree, and the expression level of KI67 and PCNA was used to indicate cell proliferation in vitro and verified using xenograft tumors in vivo.
RESULTS
KIF18B is highly expressed in colon adenocarcinoma tissues and has a negative correlation with the prognosis and tumor grade of colon adenocarcinoma. Interfering with KIF18B inhibits cell proliferation in vitro and in vivo.
CONCLUSION
KIF18B can be used as a prognostic marker for colon adenocarcinoma and may be a therapeutic target for colon adenocarcinoma treatment.
背景
驱动蛋白超家族蛋白（KIFs）在多种肿瘤中的作用已有报道，且KIFs促进癌细胞增殖。但很少有研究聚焦于结肠腺癌。
方法
通过生物信息学分析和免疫组织化学（IHC）检测，确定KIF18B在结肠腺癌组织中的表达。使用慢病毒介导的KIF18B短发夹RNA构建稳定的KIF18B缺失细胞系。进行细胞集落形成试验和CCK8试验以评估细胞增殖程度，并用KI67和PCNA的表达水平来指示体外细胞增殖情况，并在体内异种移植瘤中进行验证。
结果
KIF18B在结肠腺癌组织中高表达，且与结肠腺癌的预后和肿瘤分级呈负相关。干扰KIF18B可抑制体内外细胞增殖。
结论
KIF18B可作为结肠腺癌的预后标志物，可能是结肠腺癌治疗的一个靶点。
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