Teruya Takayuki, Goga Haruhisa, Yanagida Mitsuhiro
G0 Cell Unit Okinawa Institute of Science and Technology Graduate University Okinawa Japan.
Forensic Laboratory, Department of Criminal Investigation Okinawa Prefectural Police HQ Okinawa Japan.
FASEB Bioadv. 2020 Oct 21;2(12):720-733. doi: 10.1096/fba.2020-00047. eCollection 2020 Dec.
Metabolites in human biofluids document the physiological status of individuals. We conducted comprehensive, non-targeted, non-invasive metabolomic analysis of urine from 27 healthy human subjects, comprising 13 young adults (30 ± 3 years) and 14 seniors (76 ± 4 years). Quantitative analysis of 99 metabolites revealed 55 that displayed significant differences in abundance between the two groups. Forty-four did not show a statistically significant relationship with age. These include 13 standard amino acids, 5 methylated, 4 acetylated, and 9 other amino acids, 6 nucleosides, nucleobases, and derivatives, 4 sugar derivatives, 5 sugar phosphates, 4 carnitines, 2 hydroxybutyrates, 1 choline, and 1 ethanolamine derivative, and glutathione disulfide. Abundances of 53 compounds decreased, while 2 (glutathione disulfide, myo-inositol) increased in elderly people. The great majority of age-linked markers were highly correlated with creatinine. In contrast, 44 other urinary metabolites, including urate, carnitine, hippurate, and betaine, were not age-linked, neither declining nor increasing in elderly subjects. As metabolite profiles of urine and blood are quite different, age-related information in urine offers additional valuable insights into aging mechanisms of endocrine system. Correlation analysis of urinary metabolites revealed distinctly inter-related groups of compounds.
人体生物流体中的代谢物反映了个体的生理状态。我们对27名健康人类受试者的尿液进行了全面、非靶向、非侵入性的代谢组学分析，其中包括13名年轻人（30±3岁）和14名老年人（76±4岁）。对99种代谢物的定量分析显示，两组之间有55种代谢物的丰度存在显著差异。44种代谢物与年龄没有统计学上的显著关系。这些代谢物包括13种标准氨基酸、5种甲基化氨基酸、4种乙酰化氨基酸和9种其他氨基酸、6种核苷、核碱基及其衍生物、4种糖衍生物、5种糖磷酸、4种肉碱、2种羟基丁酸、1种胆碱、1种乙醇胺衍生物以及谷胱甘肽二硫化物。老年人中53种化合物的丰度下降，而2种（谷胱甘肽二硫化物、肌醇）的丰度增加。绝大多数与年龄相关的标志物与肌酐高度相关。相比之下，包括尿酸盐、肉碱、马尿酸盐和甜菜碱在内的其他44种尿液代谢物与年龄无关，在老年受试者中既不下降也不增加。由于尿液和血液的代谢物谱有很大不同，尿液中与年龄相关的信息为内分泌系统的衰老机制提供了额外有价值的见解。尿液代谢物的相关性分析揭示了明显相互关联的化合物组。
